Intralesional bovine papillomavirus DNA loads reflect severity of equine sarcoid disease

Haralambus, R.; Burgstaller, J.; Klukowska-Rotzler, J.; Steinborn, R.; Buchinger, S.; Gerber, V.; Brandt, S. (2010). Intralesional bovine papillomavirus DNA loads reflect severity of equine sarcoid disease. Equine veterinary journal, 42(4), pp. 327-31. Oxford: Wiley-Blackwell 10.1111/j.2042-3306.2010.00078.x

Full text not available from this repository.

REASONS FOR PERFORMING STUDY: Sarcoids are nonmetastasising, yet locally aggressive skin tumours that constitute the most frequent neoplasm in equids. Infection by bovine papillomaviruses types 1 and 2 (BPV-1, BPV-2) has been recognised as major causative factor in sarcoid pathogenesis, but a possible correlation of intralesional virus load with disease severity has not been established thus far. HYPOTHESIS: Given the pathogenic role of BPV-1 and BPV-2 in sarcoid disease, we suggest that intralesional viral DNA concentration may reflect the degree of affection. METHODS: Severity of disease was addressed by recording the tumour growth kinetics, lesion number and tumour type for 37 sarcoid-bearing horses and one donkey. Viral load was estimated via quantitative real-time PCR (qPCR) of the E2, E5, L1 and L2 genes from the BPV-1/-2 genome for one randomly selected lesion per horse and correlated with disease severity. RESULTS: Quantitative PCR against E2 identified viral DNA concentrations ranging from 0-556 copies/tumour cell. Of 16 horses affected by quiescent, slowly growing single tumours or multiple mild-type lesions, 15 showed a viral load up to 1.4 copies per cell. In stark contrast, all equids (22/22) bearing rapidly growing and/or multiple aggressive sarcoids had a viral load between 3 and 569 copies per cell. Consistent results were obtained with qPCR against E5, L1 and L2. CONCLUSIONS: While tumours of the same clinical type carried variable virus load, confirming that viral titre does not determine clinical appearance, we identified a highly significant correlation between intralesional viral load and disease severity. POTENTIAL RELEVANCE: The rapid determination of BPV viral load will give a reliable marker for disease severity and may also be considered when establishing a therapeutic strategy.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > ISME Equine Clinic Bern > ISME Equine Clinic, Internal medicine
UniBE Contributor:	Gerber, Vinzenz
ISSN:	0425-1644
Publisher:	Wiley-Blackwell
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:36
Last Modified:	05 Dec 2022 14:11
Publisher DOI:	10.1111/j.2042-3306.2010.00078.x
Web of Science ID:	000276790900011
URI:	https://boris.unibe.ch/id/eprint/14312 (FactScience: 221232)