Regional mRNA expression of key gluconeogenic enzymes in the liver of dairy cows

Van Dorland, H.A.; Bruckmaier, R.M. (2010). Regional mRNA expression of key gluconeogenic enzymes in the liver of dairy cows. Journal of animal physiology and animal nutrition, 94(4), pp. 505-8. Berlin: Blackwell Science 10.1111/j.1439-0396.2009.00935.x

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Liver tissue was collected from eight random dairy cows at a slaughterhouse to test if gene expression of pyruvate carboxylase (PC), mitochondrial phosphoenolpyruvate carboxykinase (PEPCKm) and cytosolic phosphoenolpyruvate carboxykinase (PEPCKc) is different at different locations in the liver. Obtained liver samples were analysed for mRNA expression levels of PC, PEPCKc and PEPCKm and subjected to the MIXED procedure of SAS to test for the sampled locations with cow liver as repeated subject. Additionally, the general linear model procedure (GLM) for analysis of variance was applied to test for significant differences for mRNA abundance of PEPCKm, PEPCKc and bPC between the livers. In conclusion, this study demonstrated that mRNA abundance of PC, PEPCKc and PEPCKm is not different between locations in the liver but may differ between individual cows.

Item Type:	Journal Article (Original Article)
Division/Institute:	05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Physiology
UniBE Contributor:	van Dorland, Hendrika Anette, Bruckmaier, Rupert
ISSN:	0931-2439
Publisher:	Blackwell Science
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:36
Last Modified:	05 Dec 2022 14:11
Publisher DOI:	10.1111/j.1439-0396.2009.00935.x
Web of Science ID:	000279670000011
URI:	https://boris.unibe.ch/id/eprint/14337 (FactScience: 221282)