Regional mRNA expression of key gluconeogenic enzymes in the liver of dairy cows

Van Dorland, H.A.; Bruckmaier, R.M. (2010). Regional mRNA expression of key gluconeogenic enzymes in the liver of dairy cows. Journal of animal physiology and animal nutrition, 94(4), pp. 505-8. Berlin: Blackwell Science 10.1111/j.1439-0396.2009.00935.x

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Liver tissue was collected from eight random dairy cows at a slaughterhouse to test if gene expression of pyruvate carboxylase (PC), mitochondrial phosphoenolpyruvate carboxykinase (PEPCKm) and cytosolic phosphoenolpyruvate carboxykinase (PEPCKc) is different at different locations in the liver. Obtained liver samples were analysed for mRNA expression levels of PC, PEPCKc and PEPCKm and subjected to the MIXED procedure of SAS to test for the sampled locations with cow liver as repeated subject. Additionally, the general linear model procedure (GLM) for analysis of variance was applied to test for significant differences for mRNA abundance of PEPCKm, PEPCKc and bPC between the livers. In conclusion, this study demonstrated that mRNA abundance of PC, PEPCKc and PEPCKm is not different between locations in the liver but may differ between individual cows.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Physiology

UniBE Contributor:

van Dorland, Hendrika Anette and Bruckmaier, Rupert

ISSN:

0931-2439

Publisher:

Blackwell Science

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:36

Last Modified:

21 Jan 2014 15:05

Publisher DOI:

10.1111/j.1439-0396.2009.00935.x

Web of Science ID:

000279670000011

URI:

https://boris.unibe.ch/id/eprint/14337 (FactScience: 221282)

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