Micrometer-resolution X-ray tomographic full-volume reconstruction of an intact post-mortem juvenile rat lung

Borisova, Elena; Lovric, Goran; Miettinen, Arttu; Fardin, Luca; Bayat, Sam; Larsson, Anders; Stampanoni, Marco; Schittny, Johannes C.; Schlepütz, Christian M. (2021). Micrometer-resolution X-ray tomographic full-volume reconstruction of an intact post-mortem juvenile rat lung. Histochemistry and cell biology, 155(2), pp. 215-226. Springer-Verlag https://doi.org/10.1007/s00418-020-01868-8

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In this article, we present an X-ray tomographic imaging method that is well suited for pulmonary disease studies in animal models to resolve the full pathway from gas intake to gas exchange. Current state-of-the-art synchrotron-based tomographic phase-contrast imaging methods allow for three-dimensional microscopic imaging data to be acquired non-destructively in scan times of the order of seconds with good soft tissue contrast. However, when studying multi-scale hierarchically structured objects, such as the mammalian lung, the overall sample size typically exceeds the field of view illuminated by the X-rays in a single scan and the necessity for achieving a high spatial resolution conflicts with the need to image the whole sample. Several image stitching and calibration techniques to achieve extended high-resolution fields of view have been reported, but those approaches tend to fail when imaging non-stable samples, thus precluding tomographic measurements of large biological samples, which are prone to degradation and motion during extended scan times. In this work, we demonstrate a full-volume three-dimensional reconstruction of an intact rat lung under immediate post-mortem conditions and at an isotropic voxel size of (2.75 µm)3. We present the methodology for collecting multiple local tomographies with 360° extended field of view scans followed by locally non-rigid volumetric stitching. Applied to the lung, it allows to resolve the entire pulmonary structure from the trachea down to the parenchyma in a single dataset. The complete dataset is available online (https://doi.org/10.16907/7eb141d3-11f1-47a6-9d0e-76f8832ed1b2).

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Borisova, Elena; Lovric, Goran and Schittny, Johannes

Subjects:

500 Science > 530 Physics
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0948-6143

Publisher:

Springer-Verlag

Funders:

[UNSPECIFIED] Paul Scherrer Institute
[4] Swiss National Science Foundation
[UNSPECIFIED] Alliance Campus Rhodanien
[20] Swedish Research Council

Language:

English

Submitter:

Elena Borisova

Date Deposited:

09 Jun 2020 17:33

Last Modified:

01 Mar 2021 01:31

Publisher DOI:

https://doi.org/10.1007/s00418-020-01868-8

PubMed ID:

32189111

Uncontrolled Keywords:

X-ray tomography, Fast tomography, Image reconstruction, Large volume tomography, Lung imaging

BORIS DOI:

10.7892/boris.144305

URI:

https://boris.unibe.ch/id/eprint/144305

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