Concentration in Saliva and Antibacterial Effect of Xylitol Chewing Gum: In Vivo and In Vitro Study

Cocco, Fabio; Cagetti, Maria Grazia; Majdub, Osama; Campus, Guglielmo (2020). Concentration in Saliva and Antibacterial Effect of Xylitol Chewing Gum: In Vivo and In Vitro Study. Applied Sciences, 10(8), p. 2900. MDPI 10.3390/app10082900

Xylitol 100%.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

Purpose. The saliva concentration of xylitol released from two chewing gums was recorded, the first containing xylitol as the only sweetener (100% xylitol) and the second containing only 22% of the polyol. In addition, the in vitro antibacterial effect of the two chewing gums was evaluated. Materials and Methods. The salivary concentration of Xylitol in 32 subjects was determined before and at 0.30, 1.00, 2.00, 5.00, and 10.00 min while using the chewing gums, and at 15.00, 20.00, and 25.00 min after the gums were discarded. In vitro antibacterial activity was determined on a pooled subgingival plaque sample obtained from four patients with periodontal disease. Cariogenic and periodontal bacteria were evaluated before and 15 min, 60 min, and 24 h after gum contact. Results. Using the 100% xylitol chewing gum, saliva levels increased bimodally, one peak after 30 s (1.49 ± 1.41 logμg/L) and a second one at a 10-min evaluation (1.41 ± 1.11 logμg/L); the 22% chewing gum peaked only two minute after contact (1.21 ± 1.24 logμg/L). Overall, a statistically significantly higher salivary concentration of xylitol was detected using the 100% xylitol gum. All bacteria decreased after the addition of the two chewing gums; the 100% gum achieved a greater decrease than the 22% gum. Conclusion. The use of both chewing gums increased the concentrations of xylitol in saliva, with a statistically significantly higher concentration using the 100% xylitol gum. Cariogenic and periodontal bacteria were reduced by both chewing gums; 100% xylitol gum produced the highest and longest lasting effect. This study opens up to the use of xylitol against periodontal disease.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Campus, Guglielmo Giuseppe


600 Technology > 610 Medicine & health








Guglielmo Giuseppe Campus

Date Deposited:

16 Jun 2020 08:23

Last Modified:

21 Jun 2020 02:41

Publisher DOI:


Uncontrolled Keywords:

xylitol; saliva; oral bacteria; sub-gingival plaque; antimicrobial effect; chewing gum




Actions (login required)

Edit item Edit item
Provide Feedback