Opportunities and limitations of milk mid-infrared spectra-based estimation of acetone and β-hydroxybutyrate for the prediction of metabolic stress and ketosis in dairy cows.

Caldeira, Monica O; Dan, Denisa; Neuheuser, Anna-Lena; Stürmlin, Remo; Weber, Christoph; Glauser, Daniel L; Stierli, Martin; Schuler, Urs; Moll, Juerg; Wegmann, Silvia; Bruckmaier, Rupert M.; Gross, Josef J. (2020). Opportunities and limitations of milk mid-infrared spectra-based estimation of acetone and β-hydroxybutyrate for the prediction of metabolic stress and ketosis in dairy cows. Journal of dairy research, 87(2), pp. 196-203. Cambridge University Press 10.1017/S0022029920000230

[img] Text
Caldeira MIR spectra BHB acetone JDR 2020.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (426kB) | Request a copy

Subclinical (SCK) and clinical (CK) ketosis are metabolic disorders responsible for big losses in dairy production. Although Fourier-transform mid-infrared spectrometry (FTIR) to predict ketosis in cows exposed to great metabolic stress was studied extensively, little is known about its suitability in predicting hyperketonemia using individual samples, e.g. in small dairy herds or when only few animals are at risk of ketosis. The objective of the present research was to determine the applicability of milk metabolites predicted by FTIR spectrometry in the individual screening for ketosis. In experiment 1, blood and milk samples were taken every two weeks after calving from Holstein (n = 80), Brown Swiss (n = 72) and Swiss Fleckvieh (n = 58) cows. In experiment 2, cows diagnosed with CK (n = 474) and 420 samples with blood β-hydroxybutyrate [BHB] <1.0 mmol/l were used to investigate if CK could be detected by FTIR-predicted BHB and acetone from a preceding milk control. In experiment 3, correlations between data from an in farm automatic milk analyser and FTIR-predicted BHB and acetone from the monthly milk controls were evaluated. Hyperketonemia occurred in majority during the first eight weeks of lactation. Correlations between blood BHB and FTIR-predicted BHB and acetone were low (r = 0.37 and 0.12, respectively, P < 0.0001), as well as the percentage of true positive values (11.9 and 16.6%, respectively). No association of FTIR predicted ketone bodies with the interval of milk sampling relative to CK diagnosis was found. Data obtained from the automatic milk analyser were moderately correlated with the same day FTIR-predicted BHB analysis (r = 0.61). In conclusion, the low correlations with blood BHB and the small number of true positive samples discourage the use of milk mid-infrared spectrometry analyses as the only method to predict hyperketonemia at the individual cow level.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Physiology

UniBE Contributor:

Stürmlin, Remo; Bruckmaier, Rupert and Gross, Josef Johann

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

0022-0299

Publisher:

Cambridge University Press

Language:

English

Submitter:

Josef Johann Gross

Date Deposited:

09 Jun 2020 08:28

Last Modified:

09 Jun 2020 08:28

Publisher DOI:

10.1017/S0022029920000230

PubMed ID:

32308161

Uncontrolled Keywords:

acetone ketosis mid-infrared spectrometry milk β-hydroxybutyrate

BORIS DOI:

10.7892/boris.144434

URI:

https://boris.unibe.ch/id/eprint/144434

Actions (login required)

Edit item Edit item
Provide Feedback