Functional characterization of Arabidopsis ARGONAUTE 3 in reproductive tissues

Jullien, Pauline E.; Grob, Stefan; Marchais, Antonin; Pumplin, Nathan; Chevalier, Clement; Bonnet, Diane M. V.; Otto, Caroline; Schott, Gregory; Voinnet, Olivier (2020). Functional characterization of Arabidopsis ARGONAUTE 3 in reproductive tissues (In Press). The Plant Journal Blackwell Science 10.1111/tpj.14868

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Arabidopsis encodes ten ARGONAUTE (AGO) effectors of RNA silencing, canonically loaded with either 21‐22 nucleotide (nt) long small RNAs (sRNA) to mediate post‐transcriptional‐gene‐silencing (PTGS) or 24nt sRNAs to promote RNA‐directed‐DNA‐methylation. Using full‐locus constructs, we characterized the expression, biochemical properties, and possible modes of action of AGO3. Although AGO3 arose from a recent duplication at the AGO2 locus, their expression patterns differ drastically, with AGO2 being expressed in both male and female gametes whereas AGO3 accumulates in aerial vascular terminations and specifically in chalazal seed integuments. Accordingly, AGO3 down‐regulation alters gene expression in siliques. Similar to AGO2, AGO3 binds sRNAs with a strong 5’‐adenosine bias, but unlike Arabidopsis AGO2, it binds most efficiently 24nt sRNAs. AGO3 immunoprecipitation experiments in siliques revealed that these sRNAs mostly correspond to genes and intergenic regions in a manner reflecting their respective accumulation from their loci‐of‐origin. AGO3 localizes to the cytoplasm and co‐fractionates with polysomes to possibly mediate PTGS via translation inhibition.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Epigenetics
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Jullien, Pauline Emilie and Bonnet, Diane Marie Valérie J.

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0960-7412

Publisher:

Blackwell Science

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

16 Jun 2020 14:29

Last Modified:

16 Jun 2020 14:29

Publisher DOI:

10.1111/tpj.14868

Uncontrolled Keywords:

Seeds, Arabidopsis, small RNA, Argonautes, AGO3, reproduction

BORIS DOI:

10.7892/boris.144528

URI:

https://boris.unibe.ch/id/eprint/144528

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