Automated High-Throughput Analysis of Tramadol and O-Desmethyltramadol in Dried Blood Spots.

Luginbühl, Marc; Angelova, Stanislava; Gaugler, Stefan; Längin, Andreas; Weinmann, Wolfgang (2020). Automated High-Throughput Analysis of Tramadol and O-Desmethyltramadol in Dried Blood Spots. Drug testing and analysis, 12(8), pp. 1126-1134. Wiley 10.1002/dta.2819

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The World Anti-Doping Agency (WADA) and the International Testing Agency (ITA) recently announced the development and implementation of dried blood spot (DBS) testing for routine analysis in time for the 2022 Winter Olympic and Paralympic Games in Beijing. Following the introduction of a ban on the use of Tramadol in competition in March 2019, the Union Cycliste International (UCI) started a pilot study for the manual analysis of Tramadol in DBS for anti-doping purposes. In this context, we present a fully automated LC-MS/MS-based method with automated sample preparation using a CAMAG DBS-MS 500 for the analysis of tramadol and its metabolite O-desmethyltramadol in DBS. The presented approach reduces manual handling in the laboratory to an absolute minimum, only requiring the preparation of calibration and quality control DBS cards. The method was developed, optimized, and validated before performing cross-validation with a liquid blood-based analysis method using authentic samples from forensic cases. During the validation process, the method showed an extraction efficiency of 62%, linearity r2 >0.99, accuracy and precision (within ±15% and ±20% at the LLOQ) for the determination of tramadol and O-desmethyltramadol. Method comparison in liquid blood with 26 samples showed good agreement (90±19% for tramadol and 94±14% for O-desmethyltramadol). In conclusion, automated analysis of tramadol and O-desmethyltramadol in DBS provides a fast and accurate solution for anti-doping screening. It is suited for high-throughput analysis, having a run time of about 4 min per sample. Furthermore, with the automated approach, manual sample extraction becomes obsolete.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Chemistry and Toxicology
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine

UniBE Contributor:

Längin, Andreas and Weinmann, Wolfgang

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1942-7603

Publisher:

Wiley

Language:

English

Submitter:

Antoinette Angehrn

Date Deposited:

03 Jul 2020 17:35

Last Modified:

21 May 2021 02:30

Publisher DOI:

10.1002/dta.2819

PubMed ID:

32396691

Uncontrolled Keywords:

Antidoping Dried Blood Spots Fully Automated Analysis Tramadol

BORIS DOI:

10.7892/boris.144783

URI:

https://boris.unibe.ch/id/eprint/144783

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