Kiouptsi, Klytaimnistra; Pontarollo, Giulia; Todorov, Hristo; Braun, Johannes; Jäckel, Sven; Koeck, Thomas; Bayer, Franziska; Karwot, Cornelia; Karpi, Angelica; Gerber, Susanne; Jansen, Yvonne; Wild, Philipp; Ruf, Wolfram; Daiber, Andreas; Van Der Vorst, Emiel; Weber, Christian; Döring, Yvonne; Reinhardt, Christoph (2020). Germ-free housing conditions do not affect aortic root and aortic arch lesion size of late atherosclerotic low-density lipoprotein receptor-deficient mice. Gut microbes, 11(6), pp. 1809-1823. Taylor & Francis Group 10.1080/19490976.2020.1767463
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The microbiota has been linked to the development of atherosclerosis, but the functional impact of these resident bacteria on the lesion size and cellular composition of atherosclerotic plaques in the aorta has never been experimentally addressed with the germ-free low-density lipoprotein receptor-deficient (Ldlr-/- ) mouse atherosclerosis model. Here, we report that 16 weeks of high-fat diet (HFD) feeding of hypercholesterolemic Ldlr-/- mice at germ-free (GF) housing conditions did not impact relative aortic root plaque size, macrophage content, and necrotic core area. Likewise, we did not find changes in the relative aortic arch lesion size. However, late atherosclerotic GF Ldlr-/- mice had altered inflammatory plasma protein markers and reduced smooth muscle cell content in their atherosclerotic root plaques relative to CONV-R Ldlr-/- mice. Neither absolute nor relative aortic root or aortic arch plaque size correlated with age. Our analyses on GF Ldlr-/- mice did not reveal a significant contribution of the microbiota in late aortic atherosclerosis.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Angiology |
UniBE Contributor: |
Döring, Yvonne |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
1949-0984 |
Publisher: |
Taylor & Francis Group |
Language: |
English |
Submitter: |
Maissa Jaen |
Date Deposited: |
25 Jun 2020 16:09 |
Last Modified: |
05 Dec 2022 15:39 |
Publisher DOI: |
10.1080/19490976.2020.1767463 |
PubMed ID: |
32579470 |
Uncontrolled Keywords: |
Microbiota age aortic arch aortic root atherosclerosis germ-free inflammatory markers lesion size low-density lipoprotein receptor-deficient mouse macrophages smooth muscle cells |
BORIS DOI: |
10.7892/boris.144853 |
URI: |
https://boris.unibe.ch/id/eprint/144853 |
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