Endothelial Progenitor Cell-Derived Factors Exert Neuroprotection in Cultured Cortical Neuronal Progenitor Cells.

Di Santo, Stefano; Seiler, Stefanie; Guzman, Raphael; Widmer, Hans Rudolf (2020). Endothelial Progenitor Cell-Derived Factors Exert Neuroprotection in Cultured Cortical Neuronal Progenitor Cells. Cell transplantation, 29, p. 963689720912689. Sage 10.1177/0963689720912689

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There is substantial evidence that stem and progenitor cells secrete trophic factors that have potential for repairing injured tissues. We have previously reported that the conditioned medium (CM) obtained from endothelial progenitor cells (EPC) cultures protects striatal neurons against 3-nitropropionic acid-induced toxicity. In the present study we tested the hypothesis that EPC-CM may support cortical neuronal cell function and/or survival. EPC were isolated from the peripheral blood of healthy human donors and cultured in hypoxic conditions (1.5% O2) to stimulate the secretion of growth factors. The supernatant or conditioned medium (EPC-CM) was then collected and used for the various experiments. Primary cultures of cerebral cortex from fetal rat embryonic day 14 were treated with EPC-CM and challenged by glucose and serum deprivation. We observed that EPC-CM treatment significantly increased total cell number and cell viability in the cultures. Similarly, the number of lba1-expressing cells was significantly upregulated by EPC-CM, while western blot analyses for the astroglial marker glial fibrillary acidic protein did not show a marked difference. Importantly, the number of beta-lll-tubulin-positive neurons in the cultures was significantly augmented after EPC-CM treatment. Similarly, western blot analyses for beta-III-tubulin showed significant higher signal intensities. Furthermore, EPC-CM administration protected neurons against glucose- and serum deprivation-induced cell loss. In sum, our findings identified EPC-CM as a means to promote viability and/or differentiation of cortical neurons and suggest that EPC-CM might be useful for neurorestorative approaches.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery

UniBE Contributor:

Di Santo, Stefano and Widmer, Hans Rudolf

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1555-3892

Publisher:

Sage

Language:

English

Submitter:

Nicole Söll

Date Deposited:

07 Jul 2020 15:05

Last Modified:

28 Jul 2020 13:13

Publisher DOI:

10.1177/0963689720912689

PubMed ID:

32193955

Uncontrolled Keywords:

beta-lll-tubulin cortical cultures endothelial progenitor cells neurons neuroprotection paracrine factors

BORIS DOI:

10.7892/boris.145031

URI:

https://boris.unibe.ch/id/eprint/145031

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