Analytical profiling and stability evaluation of liposomal drug delivery systems: a rapid UHPLC-CAD-based approach for phospholipids in research and quality control

Weber, Florian; Rahnfeld, Lisa; Luciani, Paola (2020). Analytical profiling and stability evaluation of liposomal drug delivery systems: a rapid UHPLC-CAD-based approach for phospholipids in research and quality control. Talanta, 220, p. 121320. Elsevier 10.1016/j.talanta.2020.121320

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Phospholipids and their derivatives represent a broad range of multifunctional substances used as excipients or active ingredients by different industries due to their natural origin and unique properties. A fast and reliable quantification as well as comprehensive stability evaluation are of major importance in the process of development and quality control of lipid-based systems. Therefore, the present study is focused on the development and validation of a rapid ultra-high performance liquid chromatography – charged aerosol detector based (UHPLC-CAD) method for simultaneous detection of a multitude of natural and synthetic lipids, (charged) phospholipids, lipophilic fluorescent markers and their possible degradation products. Twenty-two compounds were characterized by a strong linear response of the detector (R2 > 0.97). Moreover, remarkable limits of detection (≤10 μg/mL) and limits of quantification (≤25 μg/mL) associated with a consistent reproducibility were achieved for all tested molecules. The performance of the analytical method was demonstrated by analyzing the lipid composition (after different production stages and photodegradation) of both bupivacaine loaded liposomes and a Doxil®-like formulation. The newly developed method combines a rapid, comprehensive, and efficient quantification with minimal economic effort and ecologic consequences, meeting the requirements of modern analytical processes and offering a broad range of possible applications in various industrial sectors and scientific laboratories.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Weber, Florian; Rahnfeld, Lisa and Luciani, Paola

Subjects:

500 Science > 540 Chemistry
600 Technology > 610 Medicine & health > 615 Pharmacology & therapeutics, prescription drugs

ISSN:

0039-9140

Publisher:

Elsevier

Funders:

[UNSPECIFIED] Phospholipids Research Center

Language:

English

Submitter:

Paola Luciani

Date Deposited:

09 Jul 2020 17:33

Last Modified:

02 Aug 2020 12:25

Publisher DOI:

10.1016/j.talanta.2020.121320

BORIS DOI:

10.7892/boris.145102

URI:

https://boris.unibe.ch/id/eprint/145102

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