Structural Insights into the Mechanism of Mitoribosomal Large Subunit Biogenesis

Jaskolowski, Mateusz; Ramrath, David J.F.; Bieri, Philipp; Niemann, Moritz; Mattei, Simone; Calderaro, Salvatore; Leibundgut, Marc; Horn, Elke K.; Boehringer, Daniel; Schneider, André; Ban, Nenad (2020). Structural Insights into the Mechanism of Mitoribosomal Large Subunit Biogenesis. Molecular cell, 79(4), 629-644.e4. Cell Press 10.1016/j.molcel.2020.06.030

[img] Text
Molecular-Cell-Structural-Insights.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (8MB) | Request a copy

In contrast to the bacterial translation machinery, mitoribosomes and mitochondrial translation factors are highly divergent in terms of composition and architecture. There is increasing evidence that the biogenesis of mitoribosomes is an intricate pathway, involving many assembly factors. To better understand this process, we investigated native assembly intermediates of the mitoribosomal large subunit from the human parasite Trypanosoma brucei using cryo-electron microscopy. We identify 28 assembly factors, 6 of which are homologous to bacterial and eukaryotic ribosome assembly factors. They interact with the partially folded rRNA by specifically recognizing functionally important regions such as the peptidyltransferase center. The architectural and compositional comparison of the assembly intermediates indicates a stepwise modular assembly process, during which the rRNA folds toward its mature state. During the process, several conserved GTPases and a helicase form highly intertwined interaction networks that stabilize distinct assembly intermediates. The presented structures provide general insights into mitoribosomal maturation.

Item Type:

Journal Article (Original Article)


08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)
08 Faculty of Science > Other Institutions > Teaching Staff, Faculty of Science

UniBE Contributor:

Niemann, Moritz, Calderaro, Salvatore, Horn Schneider, Elke Karin, Schneider, André


500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry




Cell Press




Christina Schüpbach

Date Deposited:

21 Jul 2020 09:10

Last Modified:

02 Mar 2023 23:33

Publisher DOI:





Actions (login required)

Edit item Edit item
Provide Feedback