Structural Insights into the Mechanism of Mitoribosomal Large Subunit Biogenesis

Jaskolowski, Mateusz; Ramrath, David J.F.; Bieri, Philipp; Niemann, Moritz; Mattei, Simone; Calderaro, Salvatore; Leibundgut, Marc; Horn, Elke K.; Boehringer, Daniel; Schneider, André; Ban, Nenad (2020). Structural Insights into the Mechanism of Mitoribosomal Large Subunit Biogenesis. Molecular cell, 79(4), 629-644.e4. Cell Press 10.1016/j.molcel.2020.06.030

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In contrast to the bacterial translation machinery, mitoribosomes and mitochondrial translation factors are highly divergent in terms of composition and architecture. There is increasing evidence that the biogenesis of mitoribosomes is an intricate pathway, involving many assembly factors. To better understand this process, we investigated native assembly intermediates of the mitoribosomal large subunit from the human parasite Trypanosoma brucei using cryo-electron microscopy. We identify 28 assembly factors, 6 of which are homologous to bacterial and eukaryotic ribosome assembly factors. They interact with the partially folded rRNA by specifically recognizing functionally important regions such as the peptidyltransferase center. The architectural and compositional comparison of the assembly intermediates indicates a stepwise modular assembly process, during which the rRNA folds toward its mature state. During the process, several conserved GTPases and a helicase form highly intertwined interaction networks that stabilize distinct assembly intermediates. The presented structures provide general insights into mitoribosomal maturation.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry
08 Faculty of Science > Other Institutions > Teaching Staff, Faculty of Science

UniBE Contributor:

Niemann, Moritz; Calderaro, Salvatore; Horn, Elke and Schneider, André

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1097-2765

Publisher:

Cell Press

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

21 Jul 2020 09:10

Last Modified:

18 Sep 2020 14:30

Publisher DOI:

10.1016/j.molcel.2020.06.030

BORIS DOI:

10.7892/boris.145269

URI:

https://boris.unibe.ch/id/eprint/145269

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