Supramolecular assembly of DNA-constructed vesicles

Rothenbühler, Simon; Iacovache, Ioan; Langenegger, Simon M.; Zuber, Benoît; Häner, Robert (2020). Supramolecular assembly of DNA-constructed vesicles. Nanoscale, 12(41), pp. 21118-21123. Royal Society of Chemistry 10.1039/D0NR04103C

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The self-assembly of DNA hybrids possessing tetraphenylethylene sticky ends at both sides into vesicular architectures in aqueous medium is demonstrated. Cryo-electron microscopy reveals the formation of different types of morphologies from the amphiphilic DNA-hybrids. Depending on the conditions, either an extended (sheet-like) or a compact (columnar) alignment of the DNA hybrids is observed. The different modes of DNA arrangement lead to the formation of vesicles appearing either as prolate ellipsoids (type I) or as spheres (type II). The type of packing has a significant effect on the accessibility of the DNA, as evidenced by intercalation and light-harvesting experiments. Only the vesicles exhibiting the sheet-like DNA alignment are accessible for intercalation by ethidium bromide or for the integration of chromophore-labelled DNA via a strand exchange process. The dynamic nature of type I vesicles enables their elaboration into artificial light-harvesting complexes by DNA-guided introduction of Cy3-acceptor chromophores. DNA-constructed vesicles of the kind shown here represent versatile intermediates that are amenable to further modification for tailored nanotechnology applications.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

UniBE Contributor:

Rothenbühler, Simon; Iacovache, Mircea Ioan; Langenegger, Simon Matthias; Zuber, Benoît and Häner, Robert

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry
600 Technology > 610 Medicine & health

ISSN:

2040-3364

Publisher:

Royal Society of Chemistry

Funders:

[42] Schweizerischer Nationalfonds

Language:

English

Submitter:

Robert Häner

Date Deposited:

18 Nov 2020 17:15

Last Modified:

18 Nov 2020 17:15

Publisher DOI:

10.1039/D0NR04103C

BORIS DOI:

10.7892/boris.145278

URI:

https://boris.unibe.ch/id/eprint/145278

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