Cytokine expression in bovine PBMC cultures stimulated with Hypoderma lineatum antigens.

Cabanelas, E; Panadero, R; Baumann, A.; Alves, M. P.; Summerfield, A.; García-Dios, D; Díaz, P; Remesar, S; Fernández, G; Morrondo, M P; Díez-Baños, P; López, C M (2020). Cytokine expression in bovine PBMC cultures stimulated with Hypoderma lineatum antigens. Veterinary parasitology, 283, p. 109165. Elsevier 10.1016/j.vetpar.2020.109165

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Hypoderma antigens are involved in host inflammation and immune response, conditioning larvae survival. In this study, peripheral blood mononuclear cell (PBMC) cultures from Hypoderma sensitized and unsensitized cattle were performed to determine the effect of H. lineatum antigens and incubation time (18, 24, 48 h) on IFN-γ, TNF-α, IL-10 and IL-4 mRNA gene expression determined by RT-qPCR. TNF-α and IL-4 gene expression were higher in Hypoderma previously sensitized PBMCs, suggesting that a mixed Th1/Th2 response may play a significant role in host defence reactions against Hypoderma exhibited by previously infested cattle. Incubation time had a significant effect on IL-10 and TNF-α gene expression, which decreased over time. Regarding to H. lineatum antigens, the crude larval extract and the purified fraction hypodermin B (HB) produced a significant reduction of the mRNA expression levels of the proinflammatory cytokine, IFN-γ; moreover, the HB had a stimulating effect on the mRNA gene expression of the anti-inflammatory cytokine IL-10, demonstrating that the parasite would modulate the host defence mechanisms by avoiding harmful immune responses that would limit its survival into the host tissues.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Baumann, Arnaud; Alves, Marco and Summerfield, Artur

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0304-4017

Publisher:

Elsevier

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

26 Aug 2020 15:16

Last Modified:

30 Aug 2020 02:46

Publisher DOI:

10.1016/j.vetpar.2020.109165

PubMed ID:

32535488

Uncontrolled Keywords:

Cattle Cytokines Hypoderma PBMC RT-qPCR

BORIS DOI:

10.7892/boris.145432

URI:

https://boris.unibe.ch/id/eprint/145432

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