Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment

Binder, Florian; Ryll, René; Drewes, Stephan; Jagdmann, Sandra; Reil, Daniela; Hiltbrunner, Melanie; Rosenfeld, Ulrike M.; Imholt, Christian; Jacob, Jens; Heckel, Gerald; Ulrich, Rainer G. (2020). Spatial and Temporal Evolutionary Patterns in Puumala Orthohantavirus (PUUV) S Segment. Pathogens, 9(7) MDPI AG 10.3390/pathogens9070548

[img]
Preview
Text
pathogens-09-00548-v3.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (4MB) | Preview

The S segment of bank vole (Clethrionomys glareolus)-associated Puumala orthohantavirus (PUUV) contains two overlapping open reading frames coding for the nucleocapsid (N) and a non-structural (NSs) protein. To identify the influence of bank vole population dynamics on PUUV S segment sequence evolution and test for spillover infections in sympatric rodent species, during 2010–2014, 883 bank voles, 357 yellow-necked mice (Apodemus flavicollis), 62 wood mice
(A. sylvaticus), 149 common voles (Microtus arvalis) and 8 field voles (M. agrestis) were collected in Baden-Wuerttemberg and North Rhine-Westphalia, Germany. In total, 27.9% and 22.3% of bank voles were positive for PUUV-reactive antibodies and PUUV-specific RNA, respectively. One of eight field voles was PUUV RNA-positive, indicating a spillover infection, but none of the other species showed evidence of PUUV infection. Phylogenetic and isolation-by-distance analyses demonstrated a spatial clustering of PUUV S segment sequences. In the hantavirus outbreak years 2010 and 2012, PUUV RNA prevalence was higher in our study regions compared to non-outbreak years 2011, 2013 and 2014. NSs amino acid and nucleotide sequence types showed temporal and/or local variation, whereas the N protein was highly conserved in the NSs overlapping region and, to a lower rate, in the N alone coding part.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE)

UniBE Contributor:

Hiltbrunner, Melanie and Heckel, Gerald

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

2076-0817

Publisher:

MDPI AG

Language:

English

Submitter:

Susanne Holenstein

Date Deposited:

01 Sep 2020 16:30

Last Modified:

01 Sep 2020 16:30

Publisher DOI:

10.3390/pathogens9070548

PubMed ID:

32650456

BORIS DOI:

10.7892/boris.146071

URI:

https://boris.unibe.ch/id/eprint/146071

Actions (login required)

Edit item Edit item
Provide Feedback