A novel technique for laser-assisted revascularization: an in vitro pilot study.

Mbaidjol, Zacharia; Stoffel, Michael H.; Frenz, Martin; Constantinescu, Mihai A. (2020). A novel technique for laser-assisted revascularization: an in vitro pilot study. (In Press). Lasers in medical science Springer-Verlag 10.1007/s10103-020-03128-6

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The common limitation of surgical revascularization procedures for severe tissue ischemia due to cardiovascular diseases is the need to interrupt blood flow during the intervention. We aim to introduce a new technique that allows a sutureless, non-occlusive revascularization. A 3-step technique was developed using rabbit's aorta to simulate a side-to-side anastomosis model. It enables the creation of a bypass circuit for revascularization. The first step was the soldering of 2 vessels in a side-to-side fashion based on the laser-assisted vascular anastomosis (LAVA) principle using a diode laser emitting irradiation at 810 nm with an albumin-based solder patch between them, followed by the creation of a channel within the patch using either a holmium-doped yttrium aluminum garnet laser (Ho:YAG) at λ = 2100 nm or a xenon-chloride excimer laser (XeCl) at λ = 308 nm. Thereby, a bypass circuit was created, thus allowing a non-ischemic revascularization. The system was deemed functional when a flow was observed across the anastomosis. The highest average tensile strength recorded after side-to-side LAVA using a diode laser power of 3.2 W for 60 s was 2278.6 ± 800 mN (n = 20). The Ho:YAG laser created the channels with less tension on the anastomosis than the excimer laser. Histological analysis showed limited thermal damage and good patch-tissue adaptation. The preliminary results of this feasibility study outline the foundations for an entirely sutureless laser-assisted revascularization procedure. The next studies will evaluate the rheological parameters across the bypass circuit to optimize the post-anastomotic flow.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Plastic and Hand Surgery
04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Plastic and Hand Surgery > Plastic, Reconstructive and Aesthetic Surgery
08 Faculty of Science > Institute of Applied Physics
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy

UniBE Contributor:

Mbaïdjol Kabra, Zacharia; Stoffel, Michael Hubert; Frenz, Martin and Constantinescu, Mihai Adrian

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 620 Engineering
500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0268-8921

Publisher:

Springer-Verlag

Language:

English

Submitter:

Veronika Picha

Date Deposited:

25 Aug 2020 16:40

Last Modified:

31 Aug 2020 10:23

Publisher DOI:

10.1007/s10103-020-03128-6

PubMed ID:

32813259

Uncontrolled Keywords:

Albumin patch Blood flow Bypass surgery Side-to-side anastomosis Sutureless Tissue soldering

BORIS DOI:

10.7892/boris.146084

URI:

https://boris.unibe.ch/id/eprint/146084

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