Single-Molecule Localization of the Cardiac Voltage-Gated Sodium Channel Reveals Different Modes of Reorganization at Cardiomyocyte Membrane Domains.

Vermij, Sarah H.; Rougier, Jean-Sébastien; Agulló-Pascual, Esperanza; Rothenberg, Eli; Delmar, Mario; Abriel, Hugues (2020). Single-Molecule Localization of the Cardiac Voltage-Gated Sodium Channel Reveals Different Modes of Reorganization at Cardiomyocyte Membrane Domains. Circulation. Arrhythmia and electrophysiology, 13(7), pp. 628-639. Lippincott Williams & Wilkins 10.1161/CIRCEP.119.008241

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BACKGROUND

Mutations in the gene encoding the cardiac voltage-gated sodium channel Nav1.5 cause various cardiac arrhythmias. This variety may arise from different determinants of Nav1.5 expression between cardiomyocyte domains. At the lateral membrane and T-tubules, Nav1.5 localization and function remain insufficiently characterized.

METHODS

We used novel single-molecule localization microscopy and computational modeling to define nanoscale features of Nav1.5 localization and distribution at the lateral membrane, the lateral membrane groove, and T-tubules in cardiomyocytes from wild-type (N=3), dystrophin-deficient (mdx; N=3) mice, and mice expressing C-terminally truncated Nav1.5 (ΔSIV; N=3). We moreover assessed T-tubules sodium current by recording whole-cell sodium currents in control (N=5) and detubulated (N=5) wild-type cardiomyocytes.

RESULTS

We show that Nav1.5 organizes as distinct clusters in the groove and T-tubules which density, distribution, and organization partially depend on SIV and dystrophin. We found that overall reduction in Nav1.5 expression in mdx and ΔSIV cells results in a nonuniform redistribution with Nav1.5 being specifically reduced at the groove of ΔSIV and increased in T-tubules of mdx cardiomyocytes. A T-tubules sodium current could, however, not be demonstrated.

CONCLUSIONS

Nav1.5 mutations may site-specifically affect Nav1.5 localization and distribution at the lateral membrane and T-tubules, depending on site-specific interacting proteins. Future research efforts should elucidate the functional consequences of this redistribution.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Vermij, Sarah Helena; Rougier, Jean-Sébastien and Abriel, Hugues

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1941-3084

Publisher:

Lippincott Williams & Wilkins

Language:

English

Submitter:

Barbara Järmann-Bangerter

Date Deposited:

26 Aug 2020 14:25

Last Modified:

12 Sep 2020 04:33

Publisher DOI:

10.1161/CIRCEP.119.008241

PubMed ID:

32536203

Uncontrolled Keywords:

cardiac arrhythmias cardiomyocyte dystrophin electrophysiology membranes microscopy sodium channel

BORIS DOI:

10.7892/boris.146120

URI:

https://boris.unibe.ch/id/eprint/146120

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