Evaluation of Smear Layer Removal and Antimicrobial Efficacy of HybenX Against Enterococcus Faecalis Biofilm.

Ballal, Vasudev; Khandelwal, Deepika; Yegneswaran, Prakash Peralam; Varghese, Jothi; Al-Haj Husain, Nadin; Özcan, Mutlu (2021). Evaluation of Smear Layer Removal and Antimicrobial Efficacy of HybenX Against Enterococcus Faecalis Biofilm. European journal of prosthodontics and restorative dentistry, 29(1), pp. 6-13. Dennis Barber 10.1922/EJPRD_2054Ballal08

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To evaluate the antibacterial efficacy of HybenX, an endodontic irrigant against Enterococcus faecalis biofilm and determine its efficacy in removal of smear layer. Forty root canals were inoculated with E. faecalis for three weeks, divided into 4 groups and irrigated with: Group1: 5 ml 2.5% sodium hypochlorite; Group2: 5 ml 2% chlorhexidine gluconate; Group3: 5 ml HybenX; Group4: 5 ml distilled water (control). Dentin shavings were collected and plated on agar plates, followed by colony forming unit (CFU) determination. For smear layer removal examination, 30 single rooted teeth were instrumented and divided into 3 groups and treated with: GroupI: 5 ml of 17% EDTA; GroupII:5 ml of HybenX; and GroupIII: 5 ml of distilled water. Samples were then subjected to SEM examination. All irrigants showed a significant reduction in CFUs compared to the control group (P⟨ .001), but none compared to each other. Regarding smear layer removal in the apical third, EDTA removed smear layer more effectively than HybenX (P = .014). HybenX demonstrated good antimicrobial efficacy against E. faecalis biofilm and removed the smear layer effectively in coronal and middle third of the root canal system. HybenX can be considered as a promising irrigating agent in root canal treatment of infected teeth.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Reconstructive Dentistry and Gerodontology

UniBE Contributor:

Al-Haj Husain, Nadin


600 Technology
600 Technology > 610 Medicine & health




Dennis Barber




Tina Lauper

Date Deposited:

07 Oct 2020 14:06

Last Modified:

28 Feb 2021 01:31

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Biofilm Chlorhexidine Gluconate Enterococcus Faecalis HybenX Smear Layer





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