A Single Monoclonal Antibody against the Peanut Allergen Ara h 2 Protects against Systemic and Local Peanut Allergy.

Storni, Federico; Cabral-Miranda, Gustavo; Rösti, Elisa; Zha, Lisha; Engeroff, Paul; Zeltins, Andris; Cragg, Mark; Vogel, Monique; Bachmann, Martin F. (2020). A Single Monoclonal Antibody against the Peanut Allergen Ara h 2 Protects against Systemic and Local Peanut Allergy. International archives of allergy and immunology, 181(5), pp. 334-341. Karger 10.1159/000505917

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BACKGROUND

Peanut allergy is the most prevalent and dangerous food allergy. Peanuts consist of a large number of different allergens and peanut-allergic patients are frequently sensitized to multiple allergens. Hence, conventional desensitization approaches aim at targeting as many allergens as possible.

METHODS

The monoclonal anti-Ara h 2 antibody (mAb) was produced by hybridoma cells derived from WT BALB/c mice after immunization with a vaccine based on virus-like particles coupled to Ara h 2. BALB/c mice were sensitized intraperitoneally with peanut extract absorbed to alum and mAbs were applied i.v. Challenge was performed the next day with the whole peanut extract intravenously and via skin prick test.

RESULTS

Here we show in peanut-allergic mice that a single high-affinity mAb specific for Ara h 2 is able to block systemic and local allergic reactions induced by the complex peanut extract. We confirm in vitro binding of the mAb to the inhibitory low-affinity FcγRIIb receptor using a sensitive biosensor and demonstrate in vivo that protection was dependent on FcγRIIb.

CONCLUSION

A single mAb specific for Ara h 2 is able to improve local and systemic allergic symptoms induced by the whole allergen mixture.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Rheumatologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Rheumatologie

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Visceral Surgery

UniBE Contributor:

Storni, Federico Lorenzo; Cabral de Miranda, Gustavo; Rösti, Elisa Simona; Engeroff, Paul Simon; Vogel, Monique and Bachmann, Martin

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1018-2438

Publisher:

Karger

Language:

English

Submitter:

Lee-Anne Brand

Date Deposited:

20 Oct 2020 11:01

Last Modified:

07 Jan 2021 15:53

Publisher DOI:

10.1159/000505917

PubMed ID:

32155619

Uncontrolled Keywords:

Antibody therapy FcγRIIb dependency Food allergy

BORIS DOI:

10.7892/boris.146994

URI:

https://boris.unibe.ch/id/eprint/146994

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