Intraflagellar Transport Complex B Proteins Regulate the Hippo Effector Yap1 during Cardiogenesis.

Peralta, Marina; Ortiz Lopez, Laia; Jerabkova, Katerina; Lucchesi, Tommaso; Vitre, Benjamin; Han, Dong; Guillemot, Laurent; Dingare, Chaitanya; Sumara, Izabela; Mercader, Nadia; Lecaudey, Virginie; Delaval, Benedicte; Meilhac, Sigolène M; Vermot, Julien (2020). Intraflagellar Transport Complex B Proteins Regulate the Hippo Effector Yap1 during Cardiogenesis. Cell reports, 32(3), p. 107932. Cell Press 10.1016/j.celrep.2020.107932

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Cilia and the intraflagellar transport (IFT) proteins involved in ciliogenesis are associated with congenital heart diseases (CHDs). However, the molecular links between cilia, IFT proteins, and cardiogenesis are yet to be established. Using a combination of biochemistry, genetics, and live-imaging methods, we show that IFT complex B proteins (Ift88, Ift54, and Ift20) modulate the Hippo pathway effector YAP1 in zebrafish and mouse. We demonstrate that this interaction is key to restrict the formation of the proepicardium and the myocardium. In cellulo experiments suggest that IFT88 and IFT20 interact with YAP1 in the cytoplasm and functionally modulate its activity, identifying a molecular link between cilia-related proteins and the Hippo pathway. Taken together, our results highlight a noncanonical role for IFT complex B proteins during cardiogenesis and shed light on a mechanism of action for ciliary proteins in YAP1 regulation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy

UniBE Contributor:

Mercader Huber, Nadia Isabel

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2211-1247

Publisher:

Cell Press

Language:

English

Submitter:

Nadia Isabel Mercader Huber

Date Deposited:

11 Dec 2020 07:13

Last Modified:

11 Dec 2020 07:21

Publisher DOI:

10.1016/j.celrep.2020.107932

PubMed ID:

32698004

Uncontrolled Keywords:

BMP Hippo Intraflagellar transport (IFT) Yap1 cargiogenesis primary cilia proepicardium

BORIS DOI:

10.7892/boris.147036

URI:

https://boris.unibe.ch/id/eprint/147036

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