Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish.

García-Poyatos, Carolina; Cogliati, Sara; Calvo, Enrique; Hernansanz-Agustín, Pablo; Lagarrigue, Sylviane; Magni, Ricardo; Botos, Marius; Langa, Javier; Amati, Francesca; Vázquez, Jesús; Mercader, Nadia; Enríquez, José Antonio (2020). Scaf1 promotes respiratory supercomplexes and metabolic efficiency in zebrafish. EMBO reports, 21(7), e50287. EMBO press 10.15252/embr.202050287

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The oxidative phosphorylation (OXPHOS) system is a dynamic system in which the respiratory complexes coexist with super-assembled quaternary structures called supercomplexes (SCs). The physiological role of SCs is still disputed. Here, we used zebrafish to study the relevance of respiratory SCs. We combined immunodetection analysis and deep data-independent proteomics to characterize these structures and found similar SCs to those described in mice, as well as novel SCs including III2  + IV2 , I + IV, and I + III2  + IV2 . To study the physiological role of SCs, we generated two null allele zebrafish lines for supercomplex assembly factor 1 (scaf1). scaf1-/- fish displayed altered OXPHOS activity due to the disrupted interaction of complexes III and IV. scaf1-/- fish were smaller in size and showed abnormal fat deposition and decreased female fertility. These physiological phenotypes were rescued by doubling the food supply, which correlated with improved bioenergetics and alterations in the metabolic gene expression program. These results reveal that SC assembly by Scaf1 modulates OXPHOS efficiency and allows the optimization of metabolic resources.

Item Type:

Journal Article (Original Article)

Division/Institute:

09 Interdisciplinary Units > Microscopy Imaging Center (MIC)
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

García Poyatos, Carolina; Botos, Marius-Alexandru; Langa Oliva, Javier and Mercader Huber, Nadia Isabel

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1469-3178

Publisher:

EMBO press

Language:

English

Submitter:

Nadia Isabel Mercader Huber

Date Deposited:

11 Dec 2020 07:27

Last Modified:

11 Dec 2020 07:31

Publisher DOI:

10.15252/embr.202050287

PubMed ID:

32496654

Uncontrolled Keywords:

OXPHOS super-assembly SCAF1/COX7A2L metabolism mitochondria zebrafish

BORIS DOI:

10.7892/boris.147039

URI:

https://boris.unibe.ch/id/eprint/147039

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