Quantitative measurement of lymphatic function in mice by noninvasive near-infrared imaging of a peripheral vein.

Proulx, Steven T; Ma, Qiaoli; Andina, Diana; Leroux, Jean-Christophe; Detmar, Michael (2017). Quantitative measurement of lymphatic function in mice by noninvasive near-infrared imaging of a peripheral vein. JCI insight, 2(1), pp. 1-13. JCI Insight 10.1172/jci.insight.90861

Text b5536.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (1MB)

Optical imaging methods have been developed to measure lymphatic function in skin; however, the lymphatic system of many organs is not accessible to this technology. Since lymphatic transport of macromolecules from any organ proceeds to the blood circulation, we aimed to develop a method that can measure lymphatic function by monitoring the fluorescence in a superficial vein of an interstitially injected tracer. We selected a 40-kDa PEGylated near-infrared dye conjugate, as it showed lymphatic system-specific uptake and extended circulation in blood. Lymphatic transport to blood from subcutaneous tissue required a transit time before signal enhancement was seen in blood followed by a steady rise in signal over time. Increased lymphatic transport was apparent in awake mice compared with those under continuous anesthesia. The methods were validated in K14-VEGFR-3-Fc and K14-VEGF-C transgenic mice with loss and gain of lymphatic function, respectively. Reduced lymphatic transport to blood was also found in aged mice. The technique was also able to measure lymphatic transport from the peritoneal cavity, a location not suitable for optical imaging. The method is a promising, simple approach for assessment of lymphatic function and for monitoring of therapeutic regimens in mouse models of disease and may have potential for clinical translation.

Interest & Impact

Downloads

1 since deposited on 21 Oct 2020
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item