T Cell Migration from Inflamed Skin to Draining Lymph Nodes Requires Intralymphatic Crawling Supported by ICAM-1/LFA-1 Interactions.

Teijeira, Alvaro; Hunter, Morgan C; Russo, Erica; Proulx, Steven T; Frei, Thomas; Debes, Gudrun F; Coles, Marc; Melero, Ignacio; Detmar, Michael; Rouzaut, Ana; Halin, Cornelia (2017). T Cell Migration from Inflamed Skin to Draining Lymph Nodes Requires Intralymphatic Crawling Supported by ICAM-1/LFA-1 Interactions. Cell reports, 18(4), pp. 857-865. Cell Press 10.1016/j.celrep.2016.12.078

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T cells are the most abundant cell type found in afferent lymph, but their migration through lymphatic vessels (LVs) remains poorly understood. Performing intravital microscopy in the murine skin, we imaged T cell migration through afferent LVs in vivo. T cells entered into and actively migrated within lymphatic capillaries but were passively transported in contractile collecting vessels. Intralymphatic T cell number and motility were increased during contact-hypersensitivity-induced inflammation and dependent on ICAM-1/LFA-1 interactions. In vitro, blockade of endothelial cell-expressed ICAM-1 reduced T cell adhesion, crawling, and transmigration across lymphatic endothelium and decreased T cell advancement from capillaries into lymphatic collectors in skin explants. In vivo, T cell migration to draining lymph nodes was significantly reduced upon ICAM-1 or LFA-1 blockade. Our findings indicate that T cell migration through LVs occurs in distinct steps and reveal a key role for ICAM-1/LFA-1 interactions in this process.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute

UniBE Contributor:

Proulx, Steven Thomas

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2211-1247

Publisher:

Cell Press

Language:

English

Submitter:

Ursula Zingg-Zünd

Date Deposited:

21 Oct 2020 11:37

Last Modified:

21 Oct 2020 13:30

Publisher DOI:

10.1016/j.celrep.2016.12.078

PubMed ID:

28122237

Uncontrolled Keywords:

ICAM-1 T cell cell migration inflammation intravital microscopy lymphatic vessels

BORIS DOI:

10.7892/boris.147197

URI:

https://boris.unibe.ch/id/eprint/147197

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