A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research.

Bianchi, Roberta; Teijeira, Alvaro; Proulx, Steven T; Christiansen, Ailsa J; Seidel, Catharina D; Rülicke, Thomas; Mäkinen, Taija; Hägerling, René; Halin, Cornelia; Detmar, Michael (2015). A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research. PLoS ONE, 10(4), e0122976. Public Library of Science 10.1371/journal.pone.0122976

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The lymphatic vascular system plays an active role in immune cell trafficking, inflammation and cancer spread. In order to provide an in vivo tool to improve our understanding of lymphatic vessel function in physiological and pathological conditions, we generated and characterized a tdTomato reporter mouse and crossed it with a mouse line expressing Cre recombinase under the control of the lymphatic specific promoter Prox1 in an inducible fashion. We found that the tdTomato fluorescent signal recapitulates the expression pattern of Prox1 in lymphatic vessels and other known Prox1-expressing organs. Importantly, tdTomato co-localized with the lymphatic markers Prox1, LYVE-1 and podoplanin as assessed by whole-mount immunofluorescence and FACS analysis. The tdTomato reporter was brighter than a previously established red fluorescent reporter line. We confirmed the applicability of this animal model to intravital microscopy of dendritic cell migration into and within lymphatic vessels, and to fluorescence-activated single cell analysis of lymphatic endothelial cells. Additionally, we were able to describe the early morphological changes of the lymphatic vasculature upon induction of skin inflammation. The Prox1-Cre-tdTomato reporter mouse thus shows great potential for lymphatic research.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute

UniBE Contributor:

Proulx, Steven Thomas

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Ursula Zingg-Zünd

Date Deposited:

22 Oct 2020 10:39

Last Modified:

25 Oct 2020 02:16

Publisher DOI:

10.1371/journal.pone.0122976

PubMed ID:

25849579

BORIS DOI:

10.7892/boris.147233

URI:

https://boris.unibe.ch/id/eprint/147233

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