Multi-gene metabolic engineering of tomato plants results in increased fruit yield up to 23%

Vallarino, José G.; Kubiszewski-Jakubiak, Szymon; Ruf, Stephanie; Rößner, Margit; Timm, Stefan; Bauwe, Hermann; Carrari, Fernando; Rentsch, Doris; Bock, Ralph; Sweetlove, Lee J.; Fernie, Alisdair R. (2020). Multi-gene metabolic engineering of tomato plants results in increased fruit yield up to 23%. Scientific reports, 10(1), p. 17219. Springer Nature 10.1038/s41598-020-73709-6

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The capacity to assimilate carbon and nitrogen, to transport the resultant sugars and amino acids to sink tissues, and to convert the incoming sugars and amino acids into storage compounds in the sink tissues, are key determinants of crop yield. Given that all of these processes have the potential to co-limit growth, multiple genetic interventions in source and sink tissues, plus transport processes may be necessary to reach the full yield potential of a crop. We used biolistic combinatorial co-transformation (up to 20 transgenes) for increasing C and N flows with the purpose of increasing tomato fruit yield. We observed an increased fruit yield of up to 23%. To better explore the reconfiguration of metabolic networks in these transformants, we generated a dataset encompassing physiological parameters, gene expression and metabolite profiling on plants grown under glasshouse or polytunnel conditions. A Sparse Partial Least Squares regression model was able to explain the combination of genes that contributed to increased fruit yield. This combinatorial study of multiple transgenes targeting primary metabolism thus offers opportunities to probe the genetic basis of metabolic and phenotypic variation, providing insight into the difficulties in choosing the correct combination of targets for engineering increased fruit yield.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Molecular Plant Physiology
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Rentsch, Doris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

2045-2322

Publisher:

Springer Nature

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

17 Nov 2020 10:36

Last Modified:

13 Mar 2021 11:43

Publisher DOI:

10.1038/s41598-020-73709-6

PubMed ID:

33057137

BORIS DOI:

10.7892/boris.147388

URI:

https://boris.unibe.ch/id/eprint/147388

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