Ciliary melanin-concentrating hormone receptor 1 (MCHR1) is widely distributed in the murine CNS in a sex-independent manner.

Diniz, Giovanne B; Battagello, Daniella S; Klein, Marianne O; Bono, Bianca S M; Ferreira, Jozélia G P; Motta-Teixeira, Livia C; Duarte, Jessica C G; Presse, Françoise; Nahon, Jean-Louis; Adamantidis, Antoine; Chee, Melissa J; Sita, Luciane V; Bittencourt, Jackson C (2020). Ciliary melanin-concentrating hormone receptor 1 (MCHR1) is widely distributed in the murine CNS in a sex-independent manner. Journal of neuroscience research, 98(10), pp. 2045-2071. Wiley-Liss 10.1002/jnr.24651

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Melanin-concentrating hormone (MCH) is a ubiquitous vertebrate neuropeptide predominantly synthesized by neurons of the diencephalon that can act through two G protein-coupled receptors, called MCHR1 and MCHR2. The expression of Mchr1 has been investigated in both rats and mice, but its synthesis remains poorly described. After identifying an antibody that detects MCHR1 with high specificity, we employed immunohistochemistry to map the distribution of MCHR1 in the CNS of rats and mice. Multiple neurochemical markers were also employed to characterize some of the neuronal populations that synthesize MCHR1. Our results show that MCHR1 is abundantly found in a subcellular structure called the primary cilium, which has been associated, among other functions, with the detection of free neurochemical messengers present in the extracellular space. Ciliary MCHR1 was found in a wide range of areas, including the olfactory bulb, cortical mantle, striatum, hippocampal formation, amygdala, midline thalamic nuclei, periventricular hypothalamic nuclei, midbrain areas, and in the spinal cord. No differences were observed between male and female mice, and interspecies differences were found in the caudate-putamen nucleus and the subgranular zone. Ciliary MCHR1 was found in close association with several neurochemical markers, including tyrosine hydroxylase, calretinin, kisspeptin, estrogen receptor, oxytocin, vasopressin, and corticotropin-releasing factor. Given the role of neuronal primary cilia in sensing free neurochemical messengers in the extracellular fluid, the widespread distribution of ciliary MCHR1, and the diverse neurochemical populations who synthesize MCHR1, our data indicate that nonsynaptic communication plays a prominent role in the normal function of the MCH system.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurology

UniBE Contributor:

Adamantidis, Antoine Roger

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0360-4012

Publisher:

Wiley-Liss

Language:

English

Submitter:

Chantal Kottler

Date Deposited:

09 Nov 2020 16:16

Last Modified:

09 Nov 2020 16:16

Publisher DOI:

10.1002/jnr.24651

PubMed ID:

32530066

Uncontrolled Keywords:

GPCR MCH RRID:AB_10000342 RRID:AB_10000347 RRID:AB_10680290 RRID:AB_1079363 RRID:AB_11218957 RRID:AB_2088494 RRID:AB_2143948 RRID:AB_2143957 RRID:AB_2296529 RRID:AB_2298772 RRID:AB_2313606 RRID:AB_2336615 RRID:AB_2336819 RRID:AB_2337258 RRID:AB_2340593 RRID:AB_2534082 RRID:AB_2534102 RRID:AB_2541682 RRID:AB_2552692 RRID:AB_2556542 RRID:AB_2556546 RRID:AB_2650437 RRID:AB_2650444 RRID:AB_2650446 RRID:AB_310305 RRID:AB_477010 RRID:AB_518526 RRID:AB_572219 RRID:AB_572248 RRID:AB_572268 RRID:AB_91683 RRID:SCR_014199 neurochemical characterization olfactory integration volume transmission

BORIS DOI:

10.7892/boris.147743

URI:

https://boris.unibe.ch/id/eprint/147743

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