Rohner, Lionel; Fux, Michaela (2020). Detection of Basophils and Other Granulocytes in Induced Sputum by Flow Cytometry. Methods in molecular biology, 2163, pp. 281-291. Springer 10.1007/978-1-0716-0696-4_23
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Flow cytometry is one of the most widely used techniques for the detection of surface markers on various cells, particularly the cells of the immune system, at a single-cell resolution. Modern flow cytometers can identify rare cell population in highly heterogeneous samples. Here we present a protocol that allows a precise detection of basophils as well as eosinophils and neutrophils in induced sputum samples. The identification of sputum basophils and other granulocytes contributes to a better understanding of the cellular network that promotes and regulates inflammation of the lower respiratory tract.
Item Type: |
Journal Article (Further Contribution) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry |
UniBE Contributor: |
Rohner, Lionel, Fux, Michaela |
Subjects: |
600 Technology > 610 Medicine & health 500 Science > 570 Life sciences; biology |
ISSN: |
1940-6029 |
ISBN: |
978-1-07-160695-7 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Karin Balmer |
Date Deposited: |
23 Nov 2020 15:03 |
Last Modified: |
05 Dec 2022 15:42 |
Publisher DOI: |
10.1007/978-1-0716-0696-4_23 |
PubMed ID: |
32766984 |
Uncontrolled Keywords: |
Airway inflammation Allergy Asthma Basophils Eosinophils Flow cytometry Induced sputum Neutrophils |
BORIS DOI: |
10.7892/boris.148137 |
URI: |
https://boris.unibe.ch/id/eprint/148137 |
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