Detection of Basophils and Other Granulocytes in Induced Sputum by Flow Cytometry.

Rohner, Lionel; Fux, Michaela (2020). Detection of Basophils and Other Granulocytes in Induced Sputum by Flow Cytometry. Methods in molecular biology, 2163, pp. 281-291. Springer 10.1007/978-1-0716-0696-4_23

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Flow cytometry is one of the most widely used techniques for the detection of surface markers on various cells, particularly the cells of the immune system, at a single-cell resolution. Modern flow cytometers can identify rare cell population in highly heterogeneous samples. Here we present a protocol that allows a precise detection of basophils as well as eosinophils and neutrophils in induced sputum samples. The identification of sputum basophils and other granulocytes contributes to a better understanding of the cellular network that promotes and regulates inflammation of the lower respiratory tract.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry

UniBE Contributor:

Rohner, Lionel and Fux, Michaela

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

1940-6029

ISBN:

978-1-07-160695-7

Publisher:

Springer

Language:

English

Submitter:

Karin Balmer

Date Deposited:

23 Nov 2020 15:03

Last Modified:

23 Nov 2020 15:03

Publisher DOI:

10.1007/978-1-0716-0696-4_23

PubMed ID:

32766984

Uncontrolled Keywords:

Airway inflammation Allergy Asthma Basophils Eosinophils Flow cytometry Induced sputum Neutrophils

BORIS DOI:

10.7892/boris.148137

URI:

https://boris.unibe.ch/id/eprint/148137

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