Pellicle Modification with Casein and Mucin Does Not Promote In Vitro Bacterial Biofilm Formation.

Saads Carvalho, Thiago; Halter, Judith Elisa; Muçolli, Dea; Lussi, Adrian; Eick, Sigrun; Baumann, Tommy (2020). Pellicle Modification with Casein and Mucin Does Not Promote In Vitro Bacterial Biofilm Formation. Oral health & preventive dentistry, 18(3), pp. 475-483. Quintessenz Verlags-GmbH 10.3290/j.ohpd.a43351

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PURPOSE

During biofilm formation, bacterial species do not attach directly onto the enamel surface, but rather onto the salivary pellicle. Salivary pellicle modification with casein and mucin can hinder erosive demineralisation of the enamel, but it should also not promote bacterial adhesion. The aim of our study was to assess whether salivary pellicle modification with casein, or mucin, or a mixture of both proteins (casein and mucin) influence bacterial adhesion, biofilm diversity, metabolism and composition, or enamel demineralisation, after incubation in: (a) a single bacterial model; (b) a five-species biofilm model; or (c) biofilm reformation using the five-species biofilm model after removal of initial biofilm with toothbrushing.

MATERIALS AND METHODS

Enamel specimens were prepared from human molars. Whole-mouth stimulated human saliva was used for pellicle formation. Four pellicle modification groups were established: control (non-modified pellicle); casein - modified with 0.5% casein; mucin - modified with 0.5% mucin; casein and mucin - modified with 0.5% casein and 0.5% mucin. Bacterial adhesion, biofilm diversity, metabolic activity, biofilm mass, and demineralisation (surface hardness) of enamel were assessed after incubation in bacterial broths after 6 h or 24 h.

RESULTS

After 24 h incubation in the five-species biofilm model, the mucin group presented significantly lower biofilm mass than the control (p = 0.028) and the casein and mucin (p = 0.030) groups. No other differences between the groups were observed in any of the other experimental procedures.

CONCLUSION

Pellicle modification with casein and mucin does not promote in vitro bacterial biofilm formation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Saads Carvalho, Thiago; Muçolli, Dea; Lussi, Adrian; Eick, Sigrun and Baumann, Tommy

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1602-1622

Publisher:

Quintessenz Verlags-GmbH

Language:

English

Submitter:

Daniela Zesiger

Date Deposited:

23 Dec 2020 09:07

Last Modified:

11 Jan 2021 22:28

Publisher DOI:

10.3290/j.ohpd.a43351

PubMed ID:

32515418

Uncontrolled Keywords:

biofilms casein demineralisation enamel mucin salivary pellicle salivary pellicle modification

BORIS DOI:

10.48350/148263

URI:

https://boris.unibe.ch/id/eprint/148263

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