Neuromuscular reinnervation efficacy using a YFP model.

Woollard, A C S; Lichtman, J W; Rolfe, K; Grobbelaar, A.O. (2021). Neuromuscular reinnervation efficacy using a YFP model. Journal of plastic, reconstructive & aesthetic surgery, 74(3), pp. 569-580. Elsevier 10.1016/j.bjps.2020.10.004

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INTRODUCTION

The gold standard reconstruction for facial reanimation is the functional muscle transfer. The reinnervation of a muscle is never complete, and clinical results are variable with 20% not achieving a satisfactory outcome. We hypothesise that this may be due to a mismatch between the characteristics of the donor nerve and transferred muscle.

METHOD

81 YFP-16 and 14 YFP-H mice were studied in three intervention groups over three time periods. Two parameters were investigated: the number and surface area of reinnervated neuromuscular junctions and regenerating axons. An assessment was made of motor unit proportions.

RESULTS

All cases of nerve repair and nerve graft, the neuromuscular junctions (NMJ) were completely reinnervated by regenerating axons. The number and calibre of the regenerating axons were significantly different from controls for both intervention groups. The motor units were smaller in both intervention groups.

DISCUSSION

Reinnervation occurs after nerve repair or graft; however, the arbour was reinnervated by large numbers of much smaller axons. These axons showed some evidence of remodelling in the repair group, but not in the graft group. Neither group achieved the parameters of the control group. There were persistent qualitative changes to the morphology of both axons and junctions. Imaging documented both synkinesis and alterations that resemble those seen in ageing.

CONCLUSION

Overall, the efficacy of reinnervation is very high with all NMJ reoccupied by regenerating axons. The way small axons are remodelled is different in the nerve repairs compared with the nerve grafts.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Plastic and Hand Surgery > Plastic, Reconstructive and Aesthetic Surgery
04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Plastic and Hand Surgery

UniBE Contributor:

Grobbelaar, Adriaan Ockert

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1748-6815

Publisher:

Elsevier

Language:

English

Submitter:

Veronika Picha

Date Deposited:

16 Dec 2020 16:51

Last Modified:

03 Mar 2021 01:32

Publisher DOI:

10.1016/j.bjps.2020.10.004

PubMed ID:

33218962

Uncontrolled Keywords:

Nerve regeneration Synkinesis Targeted muscle reinnervation YFP

BORIS DOI:

10.7892/boris.148492

URI:

https://boris.unibe.ch/id/eprint/148492

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