Evaluation of 24 protocols for the production of platelet-rich fibrin.

Miron, Richard J.; Chai, Jihua; Kobayashi, Masako; Sculean, Anton; Zhang, Yufeng (2020). Evaluation of 24 protocols for the production of platelet-rich fibrin. BMC Oral Health, 20(1), p. 310. BioMed Central 10.1186/s12903-020-01299-w

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BACKGROUND

The aim of this study was to evaluate 24 protocols for the production of platelet rich fibrin (PRF) produced via horizontal centrifugation to better understand cell separation following protocols at various times and speeds.

METHODS

All protocols were compared utilizing a recent method to quantify cells in PRF in 1 mL sequential layers pipetted from the upper layer downwards until all 10 mL were harvested. In total, 960 complete blood counts (CBCs) were investigated. Both solid and liquid-based PRF protocols were investigated following 24 protocols involving 6 relative centrifugal force (RCF) values (100, 200, 400, 700, 1000 and 1200g) at 4 centrifugation times (3, 5, 8 and 12 min).

RESULTS

In general, platelets could more easily accumulate in the upper 4 layers when compared to leukocytes owing to their lower cellular density. Protocol time seemed to have a greater impact on the final cell layer separation when compared to the effect of speed. Protocols of greater than 8 min at 400g led to no leukocyte accumulation in the upper PRF layers (found specifically within the buffy coat). Protocols at or below 200g were unable to effectively accumulate platelets/leukocytes. The optimal centrifugation speed and time for solid-PRF ranged between 400 and 700g for 8 min. It was noted that variability in patient baseline platelet/leukocyte/erythrocyte counts (hematocrit) significantly affected cell layer separation. This finding was more pronounced at lower centrifugation speeds.

CONCLUSIONS

Within the investigated ranges, a protocol of 700g for 8 min presented the highest yield of platelets/leukocytes evenly distributed throughout the upper PRF layers.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Miron, Richard John; Kobayashi, Masako and Sculean, Anton

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1472-6831

Publisher:

BioMed Central

Language:

English

Submitter:

Doris Burri

Date Deposited:

01 Dec 2020 10:31

Last Modified:

16 Dec 2020 14:44

Publisher DOI:

10.1186/s12903-020-01299-w

PubMed ID:

33160335

Uncontrolled Keywords:

A-PRF Advanced platelet-rich fibrin L-PRF Leukocyte and platelet-rich fibrin i-PRF

BORIS DOI:

10.7892/boris.148680

URI:

https://boris.unibe.ch/id/eprint/148680

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