Tissue Integration and Degradation of a Porous Collagen-Based Scaffold Used for Soft Tissue Augmentation.

Caballé-Serrano, Jordi; Zhang, Sophia; Sculean, Anton; Stähli, Alexandra; Bosshardt, Dieter D. (2020). Tissue Integration and Degradation of a Porous Collagen-Based Scaffold Used for Soft Tissue Augmentation. Materials, 13(10) Molecular Diversity Preservation International MDPI 10.3390/ma13102420

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Collagen-based scaffolds hold great potential for tissue engineering, since they closely mimic the extracellular matrix. We investigated tissue integration of an engineered porous collagen-elastin scaffold developed for soft tissue augmentation. After implantation in maxillary submucosal pouches in 6 canines, cell invasion (vimentin), extracellular matrix deposition (collagen type I) and scaffold degradation (cathepsin k, tartrate-resistant acid phosphatase (TRAP), CD86) were (immuno)-histochemically evaluated. Invasion of vimentin+ cells (scattered and blood vessels) and collagen type I deposition within the pores started at 7 days. At 15 and 30 days, vimentin+ cells were still numerous and collagen type I increasingly filled the pores. Scaffold degradation was characterized by collagen loss mainly occurring around 15 days, a time point when medium-sized multinucleated cells peaked at the scaffold margin with simultaneous labeling for cathepsin k, TRAP, and CD86. Elastin was more resistant to degradation and persisted up to 90 days in form of packages well-integrated in the newly formed soft connective tissue. In conclusion, this collagen-based scaffold maintained long-enough volume stability to allow an influx of blood vessels and vimentin+ fibroblasts producing collagen type I, that filled the scaffold pores before major biomaterial degradation and collapse occurred. Cathepsin k, TRAP and CD86 appear to be involved in scaffold degradation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Caballé Serrano, Jordi; Sculean, Anton; Stähli, Alexandra Beatrice and Bosshardt, Dieter

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1996-1944

Publisher:

Molecular Diversity Preservation International MDPI

Language:

English

Submitter:

Doris Burri

Date Deposited:

15 Dec 2020 16:56

Last Modified:

15 Dec 2020 16:56

Publisher DOI:

10.3390/ma13102420

PubMed ID:

32466244

Uncontrolled Keywords:

biomaterial collagen degradation elastin immunohistochemistry scaffold tissue engineering tissue response wound healing

BORIS DOI:

10.7892/boris.148687

URI:

https://boris.unibe.ch/id/eprint/148687

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