Taurolidine Acts on Bacterial Virulence Factors and Does Not Induce Resistance in Periodontitis-Associated Bacteria-An In-Vitro Study.

Radakovic, Sabrina; Andreoli, Nicola; Schmid, Simon; Nietzsche, Sandor; Zumbrunn, Jürg; Sculean, Anton; Eick, Sigrun (2020). Taurolidine Acts on Bacterial Virulence Factors and Does Not Induce Resistance in Periodontitis-Associated Bacteria-An In-Vitro Study. Antibiotics, 9(4) MDPI 10.3390/antibiotics9040166

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The aims of the present study were: (a) to determine the mechanism of action of taurolidine against bacterial species associated with periodontal disease, and (b) to evaluate the potential development of resistance against taurolidine as compared with minocycline. After visualizing the mode of action of taurolidine by transmission electron micrographs, the interaction with most important virulence factors (lipopolysaccharide (LPS), Porphyromonas gingivalis gingipains, Aggregatibacter actinomycetemcomitans leukotoxin), was analyzed. Then, 14 clinical isolates from subgingival biofilm samples were transferred on agar plates containing subinhibitory concentrations of taurolidine or minocycline up to 50 passages. Before and after each 10 passages, minimal inhibitory concentrations (MICs) were determined. Increasing MICs were screened for efflux mechanism. Taurolidine inhibited in a concentration-dependent manner the activities of LPS and of the arginine-specific gingipains; however, an effect on A.actinomycetemcomitans leukotoxin was not detected. One P.gingivalis strain developed a resistance against taurolidine, which was probably linked with efflux mechanisms. An increase of MIC values of minocycline occurred in five of the 14 included strains after exposure to subinhibitory concentrations of the antibiotic. The present results indicate that: a) taurolidine interacts with LPS and gingipains, and b) development of resistance seems to be a rare event when using taurolidine.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology

UniBE Contributor:

Radakovic, Sabrina Myriam; Sculean, Anton and Eick, Sigrun

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2079-6382

Publisher:

MDPI

Language:

English

Submitter:

Doris Burri

Date Deposited:

15 Dec 2020 17:08

Last Modified:

15 Dec 2020 17:08

Publisher DOI:

10.3390/antibiotics9040166

PubMed ID:

32272629

Uncontrolled Keywords:

development of resistance mechanisms of action minocycline periodontal pathogens taurolidine

BORIS DOI:

10.7892/boris.148690

URI:

https://boris.unibe.ch/id/eprint/148690

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