miRNA-21 deficiency impairs alveolar socket healing in mice.

Strauss, Franz Josef; Stähli, Alexandra; Kobatake, Reiko; Tangl, Stefan; Heimel, Patrick; Apaza Alccayhuaman, Karol Alí; Schosserer, Markus; Hackl, Matthias; Grillari, Johannes; Gruber, Reinhard (2020). miRNA-21 deficiency impairs alveolar socket healing in mice. Journal of periodontology, 91(12), pp. 1664-1672. American Academy of Periodontology 10.1002/JPER.19-0567

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OBJECTIVES

MicroRNAs (miRNAs) are small noncoding RNAs demonstrated as critical post-transcriptional modulators in dental tissues and bone regeneration, particularly miR-21-5p. However, the role of miR-21-5p in the healing of alveolar sockets following tooth extraction remains unknown. In this study we evaluated the influence of miR-21-5p in the healing of alveolar socket after tooth extraction.

METHODS

Eight miR-21-5p knockout mice and eight littermate controls underwent tooth extraction of the upper right incisor. After a healing period of 14 days microCT and histological analyses were performed.

RESULTS

MicroCT analysis showed that the percentage of bone in the extraction socket was significantly higher in the control group than in the miR-21 knockout mice; either in the coronal (39.0%, CI 31.8 to 48.0 versus 23.0%, CI 17.8 to 35.2, P = 0.03) or in the middle part of the alveolar socket (56.0%, CI 50.9 to 62.5 versus 43.5% CI 28.6 to 54.6, P = 0.03). These differences were not noted in the apical part of the extraction socket. Histological analysis supported the microCT findings. Newly bone volume per tissue volume (BV/TV) was significantly higher in the control group when compared to miR-21 knockout mice, 27.4% (CI 20.6 to 32.9) versus 19.0% (CI 14.7 to 21.5, P < 0.05), respectively. Surprisingly, no evident signs of buccal bone resorption were observed in both groups.

CONCLUSION

Despite the limitation of one observation period, these findings suggest that miR-21-5p delays the early healing of alveolar socket following tooth extraction. Whether miR-21-5p is essential for healing of alveolar sockets remains to be elucidated.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Stähli, Alexandra Beatrice and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-3492

Publisher:

American Academy of Periodontology

Language:

English

Submitter:

Doris Burri

Date Deposited:

21 Dec 2020 15:00

Last Modified:

15 Mar 2021 06:19

Publisher DOI:

10.1002/JPER.19-0567

PubMed ID:

32396233

Uncontrolled Keywords:

bone regeneration mice microRNA mir-21 tooth extraction

BORIS DOI:

10.7892/boris.148740

URI:

https://boris.unibe.ch/id/eprint/148740

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