CXCL5, CXCL8, and CXCL10 regulation by bacteria and mechanical forces in periodontium.

Rath-Deschner, Birgit; Memmert, Svenja; Damanaki, Anna; de Molon, Rafael S; Nokhbehsaim, Marjan; Eick, Sigrun; Kirschneck, Christian; Cirelli, Joni A; Deschner, James; Jäger, Andreas; Nogueira, Andressa V B (2021). CXCL5, CXCL8, and CXCL10 regulation by bacteria and mechanical forces in periodontium. Annals of anatomy - Anatomischer Anzeiger, 234(151648), p. 151648. Elsevier 10.1016/j.aanat.2020.151648

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OBJECTIVE

The aim of the present study was to evaluate the expressions of CXCL5, CXCL8, and CXCL10 in periodontal cells and tissues in response to microbial signals and/or biomechanical forces.

METHODS

Human gingival biopsies from inflamed and healthy sites were used to examine the chemokine expressions and protein levels by real-time PCR and immunohistochemistry. The chemokines were also investigated in gingival biopsies from rats submitted to experimental periodontitis and/or tooth movement. Furthermore, chemokine levels were determined in human periodontal fibroblasts stimulated by the periodontopathogen Fusobacterium nucleatum and/or constant tensile forces (CTS) by real-time PCR and ELISA. Additionally, gene expressions were evaluated in periodontal fibroblasts exposed to F. nucleatum and/or CTS in the presence and absence of a MAPK inhibitor by real-time PCR.

RESULTS

Increased CXCL5, CXCL8, and CXCL10 levels were observed in human and rat gingiva from sites of inflammation as compared with periodontal health. The rat experimental periodontitis caused a significant (p<0.05) increase in alveolar bone resorption, which was further enhanced when combined with tooth movement. In vitro, F. nucleatum caused a significant upregulation of CXCL5, CXCL8, and CXCL10 at 1 day. Once the cells were exposed simultaneously to F. nucleatum and CTS, the chemokines regulation was significantly enhanced. The transcriptional findings were also observed at protein level. Pre-incubation with the MEK1/2 inhibitor significantly (p<0.05) inhibited the stimulatory actions of F. nucleatum either alone or in combination with CTS on the expression levels of CXCL5, CXCL8, and CXCL10 at 1 d.

CONCLUSIONS

Our data provide original evidence that biomechanical strain further increases the stimulatory actions of periodontal bacteria on the expressions of these chemokines. Therefore, biomechanical loading in combination with periodontal infection may lead to stronger recruitment of immunoinflammatory cells to the periodontium, which might result in an aggravation of periodontal inflammation and destruction.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Eick, Sigrun

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0940-9602

Publisher:

Elsevier

Language:

English

Submitter:

Doris Burri

Date Deposited:

29 Dec 2020 09:05

Last Modified:

28 Jan 2021 01:33

Publisher DOI:

10.1016/j.aanat.2020.151648

PubMed ID:

33221386

Uncontrolled Keywords:

Fusobacterium nucleatum Gingivitis Orthodontic tooth movement Periodontitis Periodontium

BORIS DOI:

10.48350/148765

URI:

https://boris.unibe.ch/id/eprint/148765

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