The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential.

Milia, Egle; Usai, Marianna; Szotáková, Barbora; Elstnerová, Marie; Králová, Věra; D'hallewin, Guy; Spissu, Ylenia; Barberis, Antonio; Marchetti, Mauro; Bortone, Antonella; Campanella, Vincenzo; Mastandrea, Giorgio; Langhansová, Lenka; Eick, Sigrun (2020). The Pharmaceutical Ability of Pistacia lentiscus L. Leaves Essential Oil Against Periodontal Bacteria and Candida sp. and Its Anti-Inflammatory Potential. Antibiotics, 9(6) MDPI 10.3390/antibiotics9060281

[img]
Preview
Text
antibiotics-09-00281.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

BACKGROUND

Given the increasing request for natural pharmacological molecules, this study assessed the antimicrobial capacity of Pistacia lentiscus L. essential oil (PLL-EO) obtained from the leaves of wild plants growing in North Sardinia (Italy) toward a wide range of periodontal bacteria and Candida, including laboratory and clinical isolates sp., together with its anti-inflammatory activity and safety.

METHODS

PLL-EO was screened by gas chromatography/mass spectrometry. The minimal inhibitory concentration (MIC) was determined. The anti-inflammatory activity was measured by cyclooxygenase (COX-1/2) and lipoxygenase (LOX) inhibition, while the antioxidant capacity was determined electro-chemically and by the MTT assay. The WST-1 assay was used to ascertain cytotoxicity toward four lines of oral cells.

RESULTS

According to the concentrations of terpens, PLL-EO is a pharmacologically-active phytocomplex. MICs against periodontal bacteria ranged between 3.13 and 12.5 µg/ml, while against Candida sp. they were between 6.25 and 12.5 µg/mL. Oxidation by COX-1/2 and LOX was inhibited by 80% and 20% µg/mL of the oil, respectively. Antioxidant activity seemed negligible, and no cytotoxicity arose.

CONCLUSIONS

PLL-EO exhibits a broad-spectrum activity against periodontal bacteria and Candida, with an interesting dual inhibitory capacity toward COX-2 and LOX inflammatory enzymes, and without side effects against oral cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Eick, Sigrun

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2079-6382

Publisher:

MDPI

Language:

English

Submitter:

Doris Burri

Date Deposited:

21 Dec 2020 15:43

Last Modified:

21 Dec 2020 15:43

Publisher DOI:

10.3390/antibiotics9060281

PubMed ID:

32466371

Uncontrolled Keywords:

Candida albicans Candida glabrata Oral health care products cyclooxygenase lipoxygenase medicinal herbs periodontal disease

BORIS DOI:

10.7892/boris.148771

URI:

https://boris.unibe.ch/id/eprint/148771

Actions (login required)

Edit item Edit item
Provide Feedback