Zamolo, Susanna J.; Darbre, Tamis; Reymond, Jean-Louis (2020). Transfecting tissue models with CRISPR/Cas9 plasmid DNA using peptide dendrimers. Chemical communications, 56(80), pp. 11981-11984. Royal Society of Chemistry 10.1039/D0CC04750C
|
Text
d0cc04750c.pdf - Published Version Available under License Creative Commons: Attribution-Noncommercial (CC-BY-NC). Download (2MB) | Preview |
There is currently a lack of efficient reagents to transfect cells with large plasmid DNA, which would be enabling tools for gene editing using CRISPR/Cas9 technology. Herein, we report the discovery of peptide dendrimer Z22 as a non-viral vector for transfecting large CRISPR/Cas9 pDNA into 3D-tumor spheroids with exceptionally high efficiency, low cytotoxicity and low immunogenicity.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP) |
UniBE Contributor: |
Zamolo, Susanna Joelle, Darbre, Tamis, Reymond, Jean-Louis |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
1359-7345 |
Publisher: |
Royal Society of Chemistry |
Language: |
English |
Submitter: |
Sandra Tanja Zbinden Di Biase |
Date Deposited: |
19 Jan 2021 09:36 |
Last Modified: |
05 Dec 2022 15:42 |
Publisher DOI: |
10.1039/D0CC04750C |
BORIS DOI: |
10.48350/148878 |
URI: |
https://boris.unibe.ch/id/eprint/148878 |
Actions (login required)
 |
Edit item |