Molecular pathogenesis of spontaneous abortions - Whole genome copy number analysis and expression of angiogenic factors.

Dimova, Ivanka; Rizov, Momchil; Giragosyan, Silva; Koprinarova, Miglena; Tzoneva, Dochka; Belemezova, Kalina; Hristova-Savova, Mariela; Milachich, Tanya; Djonov, Valentin Georgiev; Shterev, Atanas (2020). Molecular pathogenesis of spontaneous abortions - Whole genome copy number analysis and expression of angiogenic factors. Taiwanese journal of obstetrics & gynecology, 59(1), pp. 99-104. 10.1016/j.tjog.2019.11.015

[img]
Preview
Text
1-s2.0-S1028455919302827-main.pdf - Published Version
Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND).

Download (1MB) | Preview

OBJECTIVE

To study two major molecular alterations in spontaneous abortions (SA) with unexplained etiology - fetal genomic anomalies and the endometrial expression of main angiogenic factors VEGFA/VEGFR2 and chemokines SDF-1/CXCR4.

MATERIALS AND METHODS

Whole genome copy number analysis by arrayCGH or Next Generation Sequencing (NGS) was applied for detection of fetal genomic imbalances. The abortive decidua of SA without fetal aneuploidies was further investigated for expression levels of the abovementioned factors using real time PCR analysis. A total of 30 abortive materials were collected from spontaneous abortions after exclusion of known predisposing factors.

RESULTS

In 21 of 30 spontaneous abortions (70%), genomic anomalies were discovered by whole genome copy number analysis. Numerical anomalies were detected in 90% of aberrant cases, and in 10% - structural aberrations were revealed. An increased expression for essential factors of angiogenesis was identified in spontaneous abortions' tissues - 3.44 times for VEGFA and 10.29 times for VEGFR2. We found an average of 14 times increase in the expression levels of SDF-1 and 3.21 times for its receptor CXCR4.

CONCLUSION

We could suggest the occurrence of increased angiogenesis in SA without fetal aneuploidies, compared to the control tissues, which could lead to increased oxidative stress and fetal loss.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy

UniBE Contributor:

Djonov, Valentin Georgiev

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1875-6263

Language:

English

Submitter:

David Christian Haberthür

Date Deposited:

10 Dec 2020 17:01

Last Modified:

17 Dec 2020 09:14

Publisher DOI:

10.1016/j.tjog.2019.11.015

PubMed ID:

32039809

Uncontrolled Keywords:

Angiogenesis ArrayCGH NGS Spontaneous abortions Whole genome copy number analysis

BORIS DOI:

10.7892/boris.149207

URI:

https://boris.unibe.ch/id/eprint/149207

Actions (login required)

Edit item Edit item
Provide Feedback