Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches: a proof-of-concept study.

Grothausmann, Roman; Labode, Jonas; Hernandez-Cerdan, Pablo; Haberthür, David; Hlushchuk, Ruslan; Lobachev, Oleg; Brandenberger, Christina; Gie, Andre George; Salaets, Thomas; Toelen, Jaan; Wagner, Willi L; Mühlfeld, Christian (2021). Combination of µCT and light microscopy for generation-specific stereological analysis of pulmonary arterial branches: a proof-of-concept study. Histochemistry and cell biology, 155(2), pp. 227-239. Springer-Verlag 10.1007/s00418-020-01946-x

[img] Text
Grothausmann2020_Article_Combination.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (7MB) | Request a copy

Various lung diseases, including pulmonary hypertension, chronic obstructive pulmonary disease or bronchopulmonary dysplasia, are associated with structural and architectural alterations of the pulmonary vasculature. The light microscopic (LM) analysis of the blood vessels is limited by the fact that it is impossible to identify which generation of the arterial tree an arterial profile within a LM microscopic section belongs to. Therefore, we established a workflow that allows for the generation-specific quantitative (stereological) analysis of pulmonary blood vessels. A whole left rabbit lung was fixed by vascular perfusion, embedded in glycol methacrylate and imaged by micro-computed tomography (µCT). The lung was then exhaustively sectioned and 20 consecutive sections were collected every 100 µm to obtain a systematic uniform random sample of the whole lung. The digital processing involved segmentation of the arterial tree, generation analysis, registration of LM sections with the µCT data as well as registration of the segmentation and the LM images. The present study demonstrates that it is feasible to identify arterial profiles according to their generation based on a generation-specific color code. Stereological analysis for the first three arterial generations of the monopodial branching of the vasculature included volume fraction, total volume, lumen-to-wall ratio and wall thickness for each arterial generation. In conclusion, the correlative image analysis of µCT and LM-based datasets is an innovative method to assess the pulmonary vasculature quantitatively.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Topographical and Clinical Anatomy

UniBE Contributor:

Haberthür, David and Hlushchuk, Ruslan

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0948-6143

Publisher:

Springer-Verlag

Language:

English

Submitter:

David Christian Haberthür

Date Deposited:

17 Dec 2020 16:04

Last Modified:

01 Mar 2021 01:32

Publisher DOI:

10.1007/s00418-020-01946-x

PubMed ID:

33263790

Uncontrolled Keywords:

3D reconstruction Branching generation analysis Light microscopy Micro computed tomography Pulmonary vasculature Stereology

BORIS DOI:

10.7892/boris.149264

URI:

https://boris.unibe.ch/id/eprint/149264

Actions (login required)

Edit item Edit item
Provide Feedback