Peripheral blood CD163(+) monocytes and soluble CD163 in dry and neovascular age-related macular degeneration

Daftarian, Narsis; Zandi, Souska; Piryaie, Golbarg; Zarif, Mahin Nikougoftar; Pirmardan, Ehsan Ranaei; Yamaguchi, Muneo; Nejad, Qurban Behzadian; Hasanpour, Hossein; Samiei, Shahram; Pfister, Isabel B.; Soheili, Zahra‐Soheila; Nakao, Shintaro; Barakat, Aliaa; Garweg, Justus G.; Ahmadieh, Hamid; Hafezi‐Moghadam, Ali (2020). Peripheral blood CD163(+) monocytes and soluble CD163 in dry and neovascular age-related macular degeneration. FASEB journal, 34(6), pp. 8001-8011. Federation of American Societies for Experimental Biology 10.1096/fj.201901902RR

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Macrophages are the main infiltrating immune cells in choroidal neovascularization (CNV), a hallmark of the human wet, or neovascular age-related macular degeneration (AMD). Due to their plasticity and ability to adapt to the local microenvironment in a tissue-dependent manner, macrophages display polar functional phenotypes characterized by their cell surface markers and their cytokine profiles. We found accumulation of hemoglobin-scavenging cluster of differentiation 163 (CD163)(+) macrophages in laser-induced CNV lesions and higher expression of CD163(+) monocytes in the peripheral blood on day 7 post injury in mice. In comparison, CD80(+) macrophages did not differ with laser-injury in young or aged mice and did not significantly change in the peripheral blood of CNV mice. We examined the percentages of CD163(+), CD206(+), and CD80(+) monocytes in the peripheral blood of patients with wet AMD, patients with dry AMD, and in age-matched individuals without AMD as controls. Percentages of peripheral blood CD163(+) monocytes in both dry AMD (P < .001) and wet AMD (P < .05) were higher than in age-matched non-AMD controls, while there was no difference between the groups in the percentages of peripheral CD206(+) and CD80(+) monocytes. Further, serum level of soluble CD163 (sCD163) was elevated only in patients with wet AMD (P < .05). An examination of 40 cytokine levels across the study groups revealed that anti-VEGF treated patients with wet AMD, who showed no exudative signs on the day of blood drawing had a cytokine profile that was similar to that of non-AMD individuals. These results indicate that CD163 could be further evaluated for its potential as a useful marker of disease activity in patients with neovascular AMD. Future studies will address the origin and potential mechanistic role of CD163(+) macrophages in wet AMD pathologies of angiogenesis and leakage of blood components.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology

UniBE Contributor:

Zandi, Souska Sophie


600 Technology > 610 Medicine & health




Federation of American Societies for Experimental Biology




Souska Sophie Zandi

Date Deposited:

04 Jan 2021 09:12

Last Modified:

05 Dec 2022 15:42

Publisher DOI:


PubMed ID:





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