Platelet-Rich Fibrin Can Neutralize Hydrogen Peroxide-Induced Cell Death in Gingival Fibroblasts.

Kargarpour, Zahra; Nasirzade, Jila; Di Summa, Francesca; Panahipour, Layla; Miron, Richard J.; Gruber, Reinhard (2020). Platelet-Rich Fibrin Can Neutralize Hydrogen Peroxide-Induced Cell Death in Gingival Fibroblasts. Antioxidants, 9(6) MDPI 10.3390/antiox9060560

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Hydrogen peroxide is a damage signal at sites of chronic inflammation. The question arises whether platelet-rich fibrin (PRF), platelet-poor plasma (PPP), and the buffy coat can neutralize hydrogen peroxide toxicity and thereby counteract local oxidative stress. In the present study, gingival fibroblasts cells were exposed to hydrogen peroxide with and without lysates obtained from PRF membranes, PPP, heated PPP (75 °C for 10 min), and the buffy coat. Cell viability was examined by trypan blue staining, live-dead staining, and formazan crystal formation. Cell apoptosis was assessed by cleaved caspase-3 Western blot analysis. Reverse transcription-quantitative polymerase chain reaction (RT-PCR) was utilized to determine the impact of PRF lysates on the expression of catalase in fibroblasts. It was reported that lysates from PRF, PPP, and the buffy coat-but not heated PPP-abolished the hydrogen peroxide-induced toxicity in gingival fibroblasts. Necrosis was confirmed by a loss of membrane integrity and apoptosis was ruled out by the lack of cleavage of caspase-3. Aminotriazole, an inhibitor of catalase, reduced the cytoprotective activity of PRF lysates yet blocking of glutathione peroxidase by mercaptosuccinate did not show the same effect. PRF lysates had no impact on the expression of catalase in gingival fibroblasts. These findings suggest that PRF, PPP, and the buffy coat can neutralize hydrogen peroxide through the release of heat-sensitive catalase.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Miron, Richard John and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2076-3921

Publisher:

MDPI

Language:

English

Submitter:

Doris Burri

Date Deposited:

06 Jan 2021 11:33

Last Modified:

06 Jan 2021 11:33

Publisher DOI:

10.3390/antiox9060560

PubMed ID:

32604944

Uncontrolled Keywords:

acute toxicity apoptosis catalase dentistry oxidative stress platelet-rich-fibrin platelets ulcer wound healing

BORIS DOI:

10.48350/149748

URI:

https://boris.unibe.ch/id/eprint/149748

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