Aptamers recognizing fibronectin confer improved bioactivity to biomaterials and promote new bone formation in a periodontal defect in rats.

Parisi, Ludovica; Rivara, Federico; Costa, Camila A; Abuna, Rodriguo Pf; Palioto, Daniela B; Macaluso, Guido M (2020). Aptamers recognizing fibronectin confer improved bioactivity to biomaterials and promote new bone formation in a periodontal defect in rats. Biomedical materials, 16(1), 015016. Institute of Physics Publishing IOP 10.1088/1748-605X/abb6b2

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The use of alloplastic materials in periodontal regenerative therapies is limited by their incapacity to establish a dynamic dialog with the surrounding milieu. The aim of the present study was to control biomaterial surface bioactivity by introducing aptamers to induce the selective adsorption of fibronectin from blood, thus promoting platelets activation in vitro and bone regeneration in vivo. A hyaluronic acid/polyethyleneglycole-based hydrogel was enriched with aptamers selected for recognizing and binding fibronectin. In vitro, the capacity of constructs to support osteoblast adhesion, as well as platelets aggregation and activation was assessed by chemiluminescence within 24 h. Matrices were then evaluated in a rat periodontal defect to assess their regenerative potential by microcomputed tomography (µCT) and their osteogenic capacity by Luminex assay 5, 15 and 30 d postoperatively. Aptamers were found to confer matrices the capacity of sustaining firm cell adhesion (p = 0.0377) and to promote platelets activation (p = 0.0442). In vivo, aptamers promoted new bone formation 30 d post-operatively (p < 0.001) by enhancing osteoblastic lineage commitment maturation. Aptamers are a viable surface modification, which confers alloplastic materials the potential capacity to orchestrate blood clot formation, thus controlling bone healing.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Orthodontics

UniBE Contributor:

Parisi, Ludovica

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1748-6041

Publisher:

Institute of Physics Publishing IOP

Language:

English

Submitter:

Renate Imhof-Etter

Date Deposited:

07 Jan 2021 16:41

Last Modified:

10 Jan 2021 02:52

Publisher DOI:

10.1088/1748-605X/abb6b2

PubMed ID:

33325378

BORIS DOI:

10.48350/149854

URI:

https://boris.unibe.ch/id/eprint/149854

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