False-negative results of initial RT-PCR assays for COVID-19: A systematic review.

Arevalo-Rodriguez, Ingrid; Buitrago-Garcia, Diana; Simancas-Racines, Daniel; Zambrano-Achig, Paula; Del Campo, Rosa; Ciapponi, Agustin; Sued, Omar; Martinez-García, Laura; Rutjes, Anne; Low, Nicola; Bossuyt, Patrick M; Perez-Molina, Jose A; Zamora, Javier (2020). False-negative results of initial RT-PCR assays for COVID-19: A systematic review. PLoS ONE, 15(12), e0242958. Public Library of Science 10.1371/journal.pone.0242958

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BACKGROUND

A false-negative case of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is defined as a person with suspected infection and an initial negative result by reverse transcription-polymerase chain reaction (RT-PCR) test, with a positive result on a subsequent test. False-negative cases have important implications for isolation and risk of transmission of infected people and for the management of coronavirus disease 2019 (COVID-19). We aimed to review and critically appraise evidence about the rate of RT-PCR false-negatives at initial testing for COVID-19.

METHODS

We searched MEDLINE, EMBASE, LILACS, as well as COVID-19 repositories, including the EPPI-Centre living systematic map of evidence about COVID-19 and the Coronavirus Open Access Project living evidence database. Two authors independently screened and selected studies according to the eligibility criteria and collected data from the included studies. The risk of bias was assessed using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool. We calculated the proportion of false-negative test results using a multilevel mixed-effect logistic regression model. The certainty of the evidence about false-negative cases was rated using the GRADE approach for tests and strategies. All information in this article is current up to July 17, 2020.

RESULTS

We included 34 studies enrolling 12,057 COVID-19 confirmed cases. All studies were affected by several risks of bias and applicability concerns. The pooled estimate of false-negative proportion was highly affected by unexplained heterogeneity (tau-squared = 1.39; 90% prediction interval from 0.02 to 0.54). The certainty of the evidence was judged as very low due to the risk of bias, indirectness, and inconsistency issues.

CONCLUSIONS

There is substantial and largely unexplained heterogeneity in the proportion of false-negative RT-PCR results. The collected evidence has several limitations, including risk of bias issues, high heterogeneity, and concerns about its applicability. Nonetheless, our findings reinforce the need for repeated testing in patients with suspicion of SARS-Cov-2 infection given that up to 54% of COVID-19 patients may have an initial false-negative RT-PCR (very low certainty of evidence).

SYSTEMATIC REVIEW REGISTRATION

Protocol available on the OSF website: https://tinyurl.com/vvbgqya.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Social and Preventive Medicine (ISPM)

Graduate School:

Graduate School for Health Sciences (GHS)

UniBE Contributor:

Buitrago Garcia, Diana Carolina; Rutjes, Anne and Low, Nicola

Subjects:

600 Technology > 610 Medicine & health
300 Social sciences, sociology & anthropology > 360 Social problems & social services

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Doris Kopp Heim

Date Deposited:

21 Dec 2020 13:47

Last Modified:

06 Jan 2021 14:27

Publisher DOI:

10.1371/journal.pone.0242958

PubMed ID:

33301459

BORIS DOI:

10.7892/boris.149978

URI:

https://boris.unibe.ch/id/eprint/149978

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