Comparative proteomics of three Giardia lamblia strains: investigation of antigenic variation in the post-genomic era.

Müller, Joachim; Braga, Sophie; Uldry, Anne-Christine; Heller, Manfred; Müller, Norbert (2020). Comparative proteomics of three Giardia lamblia strains: investigation of antigenic variation in the post-genomic era. Parasitology, 147(9), pp. 1008-1018. Cambridge University Press 10.1017/S0031182020000657

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Giardia lamblia is a causative agent of persistent diarrhoea widespread in regions with low hygienic standards. Laboratory research is based on cloned lines issuing from various patient isolates typed in the late 1980s and 90s using restriction analysis and serology. In the present study, we compared the well-characterized strain WBC6 with another clone of the parent WB isolate termed WBA1 and with a clone from another isolate, GS/M-83-H7, using shotgun mass spectrometry proteomics. We identified 398 proteins differentially expressed between the GS and both WB isolates and 97 proteins differentially expressed between the two WB isolates. We investigated the expression levels of the predominant variant-specific surface proteins (VSPs) in each clone and matched the previously described major VSPs of each strain to the corresponding open reading frame sequences identified by whole-genome sequencing efforts. Furthermore, since the original WB isolate comes from a patient treated with metronidazole, we compared the susceptibilities of the strains to nitro compounds, as well the expression levels of enzymes involved in nitro reduction and on the corresponding enzyme activities and found distinct differences between the three strains.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DCR Services > Core Facility Massenspektrometrie- und Proteomics-Labor
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Protein- und Zellbiologie

UniBE Contributor:

Müller, Joachim; Braga, Sophie Marie-Pierre; Uldry, Anne-Christine; Heller, Manfred and Müller, Norbert

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0031-1820

Publisher:

Cambridge University Press

Language:

English

Submitter:

Marla Rittiner

Date Deposited:

28 Dec 2020 17:42

Last Modified:

12 Mar 2021 18:32

Publisher DOI:

10.1017/S0031182020000657

PubMed ID:

32338227

Uncontrolled Keywords:

Diversity drug susceptibility isolates parasite–host interaction

BORIS DOI:

10.48350/150163

URI:

https://boris.unibe.ch/id/eprint/150163

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