Impact of porcine cytomegalovirus on long-term orthotopic cardiac xenotransplant survival.

Denner, Joachim; Längin, Matthias; Reichart, Bruno; Krüger, Luise; Fiebig, Uwe; Mokelke, Maren; Radan, Julia; Mayr, Tanja; Milusev, Anastasia; Luther, Fabian; Sorvillo, Nicoletta; Rieben, Robert; Brenner, Paolo; Walz, Christoph; Wolf, Eckhard; Roshani, Berit; Stahl-Hennig, Christiane; Abicht, Jan-Michael (2020). Impact of porcine cytomegalovirus on long-term orthotopic cardiac xenotransplant survival. Scientific reports, 10(1), p. 17531. Springer Nature 10.1038/s41598-020-73150-9

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Xenotransplantation using pig organs has achieved survival times up to 195 days in pig orthotopic heart transplantation into baboons. Here we demonstrate that in addition to an improved immunosuppressive regimen, non-ischaemic preservation with continuous perfusion and control of post-transplantation growth of the transplant, prevention of transmission of the porcine cytomegalovirus (PCMV) plays an important role in achieving long survival times. For the first time we demonstrate that PCMV transmission in orthotopic pig heart xenotransplantation was associated with a reduced survival time of the transplant and increased levels of IL-6 and TNFα were found in the transplanted baboon. Furthermore, high levels of tPA-PAI-1 complexes were found, suggesting a complete loss of the pro-fibrinolytic properties of the endothelial cells. These data show that PCMV has an important impact on transplant survival and call for elimination of PCMV from donor pigs.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Handchirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Herz und Gefässe
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50

UniBE Contributor:

Milusev, Anastasia; Luther, Fabian Urs; Sorvillo, Nicoletta and Rieben, Robert

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2045-2322

Publisher:

Springer Nature

Language:

English

Submitter:

Marla Rittiner

Date Deposited:

29 Dec 2020 09:17

Last Modified:

03 Jan 2021 02:54

Publisher DOI:

10.1038/s41598-020-73150-9

PubMed ID:

33067513

BORIS DOI:

10.7892/boris.150167

URI:

https://boris.unibe.ch/id/eprint/150167

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