Correlative Magnetic Resonance Imaging and Histopathology in Small Ruminant Listeria Rhombencephalitis

Precht, Christina; Vermathen, Peter; Henke, Diana; Staudacher, Anne; Lauper, Josiane; Seuberlich, Torsten; Oevermann, Anna; Schweizer-Gorgas, Daniela (2020). Correlative Magnetic Resonance Imaging and Histopathology in Small Ruminant Listeria Rhombencephalitis. Frontiers in neurology, 11(518697), p. 518697. Frontiers Media S.A. 10.3389/fneur.2020.518697

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Background: Listeria rhombencephalitis, infection of the brainstem with Listeria
monocytogenes, occurs mainly in humans and farmed ruminants and is associated
with high fatality rates. Small ruminants (goats and sheep) are a large animal model
due to neuropathological similarities. The purpose of this study was to define magnetic resonance imaging (MRI) features of listeria rhombencephalitis in naturally infected small ruminants and correlate them with histopathology. Secondly, the purpose of this study was to compare the results with MRI findings reported in humans.
Methods: Twenty small ruminants (13 sheep and 7 goats) with listeria rhombencephalitis were prospectively enrolled and underwent in vivo MRI of the brain, including T2-weighted, fluid attenuation inversion recovery, and T1-weighted sequences pre- and post-contrast administration and postmortem histopathology. In MRI, lesions were characterized by location, extent, border definition, signal intensity, and contrast enhancement. In histopathology, the location, cell type, severity, and chronicity of inflammatory infiltrates and signs of vascular damage were recorded. In addition, histopathologic slides were matched to MRIs, and histopathologic and MRI features were compared.
Results: Asymmetric T2-hyperintense lesions in the brainstem were observed in all
animals and corresponded to the location and pattern of inflammatory infiltrates in
histopathology. Contrast enhancement in the brainstem was observed in 10 animals and was associated with vessel wall damage and perivascular fibrin accumulation in 8 of 10 animals. MRI underestimated the extension into rostral brain parts and the involvement of trigeminal ganglia and meninges.
Conclusion: Asymmetric T2-hyperintense lesions in the brainstem with or without contrast enhancement can be established as criteria for the diagnosis of listeria rhombencephalitis in small ruminants. Brainstem lesions were similar to human listeria rhombencephalitis in terms of signal intensity and location. Different from humans, contrast enhancement was a rare finding, and abscessation was not observed.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Clinical Radiology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Clinical Neurology
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Clinic for Ruminants
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Precht, Maria Christina; Vermathen, Peter; Seuberlich, Torsten; Oevermann, Anna and Schweizer, Daniela Esther

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

1664-2295

Publisher:

Frontiers Media S.A.

Funders:

[UNSPECIFIED] Swiss National Science Foundation

Language:

English

Submitter:

Maria Christina Precht

Date Deposited:

06 Jan 2021 15:21

Last Modified:

10 Mar 2021 21:56

Publisher DOI:

10.3389/fneur.2020.518697

PubMed ID:

33391140

Uncontrolled Keywords:

contrast enhancement, goat, sheep, listeria rhombencephalitis, listeriosis, Listeria monocytogenes

BORIS DOI:

10.48350/150836

URI:

https://boris.unibe.ch/id/eprint/150836

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