Microglial Activation in the Right Amygdala-Entorhinal-Hippocampal Complex is Associated with Preserved Spatial Learning in AppNL-G-F mice.

Biechele, Gloria; Wind, Karin; Blume, Tanja; Sacher, Christian; Beyer, Leonie; Eckenweber, Florian; Franzmeier, Nicolai; Ewers, Michael; Zott, Benedikt; Lindner, Simon; Gildehaus, Franz-Josef; von Ungern-Sternberg, Barbara; Tahirovic, Sabina; Willem, Michael; Bartenstein, Peter; Cumming, Paul; Rominger, Axel; Herms, Jochen; Brendel, Matthias (2021). Microglial Activation in the Right Amygdala-Entorhinal-Hippocampal Complex is Associated with Preserved Spatial Learning in AppNL-G-F mice. NeuroImage, 230, p. 117707. Elsevier 10.1016/j.neuroimage.2020.117707

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BACKGROUND

In Alzheimer`s disease (AD), regional heterogeneity of β-amyloid burden and microglial activation of individual patients is a well-known phenomenon. Recently, we described a high incidence of inter-individual regional heterogeneity in terms of asymmetry of plaque burden and microglial activation in β-amyloid mouse models of AD as assessed by positron-emission-tomography (PET). We now investigate the regional associations between amyloid plaque burden, microglial activation, and impaired spatial learning performance in transgenic mice in vivo.

METHODS

In 30 AppNL-G-F mice (15 female, 15 male) we acquired cross-sectional 18 kDa translocator protein (TSPO-PET, 18F-GE-180) and β-amyloid-PET (18F-florbetaben) scans at ten months of age. Control data were obtained from age- and sex-matched C57BI/6 wild-type mice. We assessed spatial learning (i.e. Morris water maze) within two weeks of PET scanning and correlated the principal component of spatial learning performance scores with voxel-wise β-amyloid and TSPO tracer uptake maps in AppNL-G-F mice, controlled for age and sex. In order to assess the effects of hemispheric asymmetry, we also analyzed correlations of spatial learning performance with tracer uptake in bilateral regions of interest for frontal cortex, entorhinal/piriform cortex, amygdala, and hippocampus, using a regression model. We tested the correlation between regional asymmetry of PET biomarkers with individual spatial learning performance.

RESULTS

Voxel-wise analyses in AppNL-G-F mice revealed that higher TSPO-PET signal in the amygdala, entorhinal and piriform cortices, the hippocampus and the hypothalamus correlated with spatial learning performance. Region-based analysis showed significant correlations between TSPO expression in the right entorhinal/piriform cortex and the right amygdala and spatial learning performance, whereas there were no such correlations in the left hemisphere. Right lateralized TSPO expression in the amygdala predicted better performance in the Morris water maze (β = -0.470, p = 0.013), irrespective of the global microglial activation and amyloid level. Region-based results for amyloid-PET showed no significant associations with spatial learning.

CONCLUSION

Elevated microglial activation in the right amygdala-entorhinal-hippocampal complex of AppNL-G-F mice is associated with better spatial learning. Our findings support a protective role of microglia on cognitive function when they highly express TSPO in specific brain regions involved in spatial memory.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Clinic of Nuclear Medicine

UniBE Contributor:

Cumming, Paul and Rominger, Axel Oliver

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1053-8119

Publisher:

Elsevier

Language:

English

Submitter:

Sabine Lanz

Date Deposited:

20 Jan 2021 14:40

Last Modified:

15 Apr 2021 01:33

Publisher DOI:

10.1016/j.neuroimage.2020.117707

PubMed ID:

33385560

Uncontrolled Keywords:

App(NL-G-F) asymmetry microglia regional heterogeneity spatial learning β-amyloid

BORIS DOI:

10.48350/150859

URI:

https://boris.unibe.ch/id/eprint/150859

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