VivoFollow 2: Distortion-Free Multiphoton Intravital Imaging

Vladymyrov, Mykhailo; Haghayegh Jahromi, Neda; Kaba, Elisa; Engelhardt, Britta; Ariga, Akitaka (2020). VivoFollow 2: Distortion-Free Multiphoton Intravital Imaging. Frontiers in physics, 7 Frontiers Media 10.3389/fphy.2019.00222

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Intravital multiphoton microscopy has become one of the central tools used in the investigation of dynamic cellular activity and function in living animals under nearly physiological conditions and is particularly important for studying the dynamic immune system. During intravital imaging in mice, periodic motion of tissue caused by respiration, induces significant shifts of the imaged region. In slow laser scanning imaging modalities, such as multiphoton microscopy, this movement can lead to considerable distortion and discontinuity in three dimensions of the acquired images. Here, we introduce VivoFollow 2, a toolkit that concurrent with image acquisition performs a precise measurement of the respective image distortion, enabling subsequent automated correction of the imaging data. Recovery of one three-dimensional image stack, corresponding to the tomographic tissue sectioning by the optical plane from each single raw image stack, preserves the time continuity within each image stack. Implementation of VivoFollow 2 thus enables a minimization of motion artifacts in tissues exposed to periodic movements and allows for long-term time-lapse imaging and subsequent precise image analysis of the dynamics of cellular and humoral factors in vivo.

Item Type:

Journal Article (Original Article)


08 Faculty of Science > Department of Mathematics and Statistics > Institute of Mathematics
04 Faculty of Medicine > Pre-clinic Human Medicine > Theodor Kocher Institute
10 Strategic Research Centers > Albert Einstein Center for Fundamental Physics (AEC)
08 Faculty of Science > Physics Institute > Laboratory for High Energy Physics (LHEP)

UniBE Contributor:

Vladymyrov, Mykhailo; Haghayegh Jahromi, Neda; Kaba, Elisa; Engelhardt, Britta and Ariga, Akitaka


600 Technology > 610 Medicine & health
500 Science > 510 Mathematics
500 Science > 530 Physics
500 Science > 570 Life sciences; biology




Frontiers Media




Ursula Zingg-Zünd

Date Deposited:

15 Jan 2021 10:19

Last Modified:

11 Mar 2021 13:11

Publisher DOI:





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