Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells

Chippalkatti, Rohan; Egger, Boris; Suter, Beat (2020). Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells. PLoS genetics, 16(11), e1008913. Public Library of Science 10.1371/journal.pgen.1008913

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Mitotic divisions depend on the timely assembly and proper orientation of the mitotic spindle. Malfunctioning of these processes can considerably delay mitosis, thereby compromising tissue growth and homeostasis, and leading to chromosomal instability. Loss of functional Mms19 drastically affects the growth and development of mitotic tissues in Drosophila lar- vae and we now demonstrate that Mms19 is an important factor that promotes spindle and astral microtubule (MT) growth, and MT stability and bundling. Mms19 function is needed for the coordination of mitotic events and for the rapid progression through mitosis that is characteristic of neural stem cells. Surprisingly, Mms19 performs its mitotic activities through two different pathways. By stimulating the mitotic kinase cascade, it triggers the localization of the MT regulatory complex TACC/Msps (Transforming Acidic Coiled Coil/Min- ispindles, the homolog of human ch-TOG) to the centrosome. This activity of Mms19 can be rescued by stimulating the mitotic kinase cascade. However, other aspects of the Mms19 phenotypes cannot be rescued in this way, pointing to an additional mechanism of Mms19 action. We provide evidence that Mms19 binds directly to MTs and that this stimulates MT stability and bundling.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Drosophila
08 Faculty of Science > Department of Biology > Institute of Cell Biology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Chippalkatti, Rohan and Suter, Beat

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1553-7390

Publisher:

Public Library of Science

Funders:

[4] Swiss National Science Foundation ; [UNSPECIFIED] University of Bern

Language:

English

Submitter:

Beat Suter

Date Deposited:

21 Jan 2021 15:40

Last Modified:

02 Mar 2022 11:57

Publisher DOI:

10.1371/journal.pgen.1008913

PubMed ID:

33211700

BORIS DOI:

10.48350/151148

URI:

https://boris.unibe.ch/id/eprint/151148

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