Sampling Method Affects HR-MAS NMR Spectra of Healthy Caprine Brain Biopsies

Häni, Annakatrin; Diserens, Gaëlle; Oevermann, Anna; Vermathen, Peter; Precht, Christina (2021). Sampling Method Affects HR-MAS NMR Spectra of Healthy Caprine Brain Biopsies. Metabolites, 11(1), p. 38. MDPI 10.3390/metabo11010038

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The metabolic profiling of tissue biopsies using high-resolution–magic angle spinning
(HR-MAS) 1H nuclear magnetic resonance (NMR) spectroscopy may be influenced by experimental factors such as the sampling method. Therefore, we compared the effects of two different sampling methods on the metabolome of brain tissue obtained from the brainstem and thalamus of healthy goats by 1H HR-MAS NMR spectroscopy—in vivo-harvested biopsy by a minimally invasive stereotactic approach compared with postmortem-harvested sample by dissection with a scalpel. Lactate and creatine were elevated, and choline-containing compounds were altered in the postmortem compared to the in vivo-harvested samples, demonstrating rapid changes most likely due to sample ischemia. In addition, in the brainstem samples acetate and inositols, and in the thalamus samples ƴ-aminobutyric acid, were relatively increased postmortem, demonstrating regional differences in tissue degradation. In conclusion, in vivo-harvested brain biopsies show different metabolic alterations compared to postmortem-harvested samples, reflecting less tissue degradation. Sampling method and brain region should be taken into account in the analysis of metabolic profiles. To be as close as possible to the actual situation in the living individual, it is desirable to use brain samples obtained by stereotactic biopsy whenever possible.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Clinical Radiology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pavillon 52 > Abt. Magnetresonanz-Spektroskopie und Methodologie, AMSM

UniBE Contributor:

Häni, Anna Katrin; Oevermann, Anna; Vermathen, Peter and Precht, Maria Christina

Subjects:

600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

2218-1989

Publisher:

MDPI

Submitter:

Maria Christina Precht

Date Deposited:

11 Jan 2021 10:20

Last Modified:

11 Jan 2021 10:20

Publisher DOI:

10.3390/metabo11010038

PubMed ID:

33419191

BORIS DOI:

10.48350/151153

URI:

https://boris.unibe.ch/id/eprint/151153

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