Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapause

Fan, Rui; Kim, Yung Su; Wu, Jie; Chen, Rui; Zeuschner, Dagmar; Mildner, Karina; Adachi, Kenjiro; Wu, Guangming; Galatidou, Styliani; Li, Jianhua; Schöler, Hans R.; Leidel, Sebastian A.; Bedzhov, Ivan (2020). Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapause. Nature Communications, 11(1) Springer Nature 10.1038/s41467-020-19353-0

[img]
Preview
Text
s41467-020-19353-0.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (10MB) | Preview

The epiblast, which provides the foundation of the future body, is actively reshaped during early embryogenesis, but the reshaping mechanisms are poorly understood. Here, using a 3D in vitro model of early epiblast development, we identify the canonical Wnt/β-catenin pathway and its central downstream factor Esrrb as the key signalling cascade regulating the tissue-scale organization of the murine pluripotent lineage. Although in vivo the Wnt/β-catenin/Esrrb circuit is dispensable for embryonic development before implantation, autocrine Wnt activity controls the morphogenesis and long-term maintenance of the epiblast when development is put on hold during diapause. During this phase, the progressive changes in the epiblast architecture and Wnt signalling response show that diapause is not a stasis but instead is a dynamic process with underlying mechanisms that can appear redundant during transient embryogenesis.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Wu, Jie and Leidel, Sebastian Andreas

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

2041-1723

Publisher:

Springer Nature

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

03 Feb 2021 14:57

Last Modified:

15 Mar 2021 11:42

Publisher DOI:

10.1038/s41467-020-19353-0

BORIS DOI:

10.48350/151555

URI:

https://boris.unibe.ch/id/eprint/151555

Actions (login required)

Edit item Edit item
Provide Feedback