Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro.

Tamò, Luca; Fytianos, Kleanthis; Caldana, Fabienne; Simillion, Cedric; Feki, Anis; Nita, Izabela; Heller, Manfred; Geiser, Thomas; Gazdhar, Amiq (2021). Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro. International journal of molecular sciences, 22(2) MDPI 10.3390/ijms22020958

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Induced pluripotent stem cell secretome (iPSC-CM) mitigate organ injury and help in repair. Macrophages play a critical role in tissue repair and regeneration and can be directed to promote tissue repair by iPSC-CM, although the exact mechanisms are not known. In the current investigative study, we evaluated the possible mechanism by which iPSC-CM regulates the phenotype and secretory pattern of macrophages in vitro. Macrophages were obtained from human peripheral blood mononuclear cells and differentiated to various subpopulations and treated with either iPSC-CM or control media in vitro. Macrophage phenotype was assessed by flow cytometry, gene expression changes by qRT PCR and secretory pattern by multiplex protein analysis. The protein and gene interaction network revealed the involvement of Amyloid precursor protein (APP) and ELAV-like protein 1 (ELAVL-1) both present in the iPSC-CM to play an important role in regulating the macrophage phenotype and their secretory pattern. This exploratory study reveals, in part, the possible mechanism and identifies two potential targets by which iPSC-CM regulate macrophages and help in repair and regeneration.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)

UniBE Contributor:

Tamò, Luca Giuseppe Athos; Fytianos, Kleanthis; Heller, Manfred; Geiser, Thomas and Gazdhar, Amiq

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1422-0067

Publisher:

MDPI

Language:

English

Submitter:

Heidi Lobsiger

Date Deposited:

16 Feb 2021 11:10

Last Modified:

16 Feb 2021 11:10

Publisher DOI:

10.3390/ijms22020958

PubMed ID:

33478018

Uncontrolled Keywords:

induced pluripotent stem cells lung fibrosis lung repair and regeneration macrophages stem cells secretome

BORIS DOI:

10.48350/151857

URI:

https://boris.unibe.ch/id/eprint/151857

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